Evidence map›Paper›PMID 31392208›Full record

ArticleBioMed research international2019

Imaging of Mouse Brain Fixated in Ethanol in Micro-CT.

Jana Mrzílková, Matěj Patzelt, Pasquale Gallina, Zdeněk Wurst, Martin Šeremeta, Jan Dudák, František Krejčí, Jan Žemlička, Vladimír Musil, Jakub Karch and 2 more

Open access · hybridAbstract read
In one paragraph

Article in BioMed research international, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.9field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 2 countries.

Jana MrzílkováSpecialized Laboratory of Experimental Imaging Third Faculty of Medicine, Charles University, Institute of Experimental and Applied Physics and Faculty of Biomedical Engineering, Czech Technical University in Prague, Prague, Czech Republic.ORCID https://orcid.org/0000-0002-9474-5040
Matěj PatzeltSpecialized Laboratory of Experimental Imaging Third Faculty of Medicine, Charles University, Institute of Experimental and Applied Physics and Faculty of Biomedical Engineering, Czech Technical University in Prague, Prague, Czech Republic.ORCID https://orcid.org/0000-0001-8269-5499
Pasquale GallinaDepartment of Surgery and Translational Medicine, Neurosurgery Unit, Florence School of Neurosurgery, University of Florence, Florence, Italy.
Zdeněk WurstDepartment of Anatomy, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
Martin ŠeremetaDepartment of Anatomy, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
Jan DudákInstitute of Experimental and Applied Physics, Czech Technical University, Prague, Czech Republic.
František KrejčíInstitute of Experimental and Applied Physics, Czech Technical University, Prague, Czech Republic.
Jan ŽemličkaInstitute of Experimental and Applied Physics, Czech Technical University, Prague, Czech Republic.
Vladimír MusilSpecialized Laboratory of Experimental Imaging Third Faculty of Medicine, Charles University, Institute of Experimental and Applied Physics and Faculty of Biomedical Engineering, Czech Technical University in Prague, Prague, Czech Republic.ORCID https://orcid.org/0000-0002-0107-690X
Jakub KarchInstitute of Experimental and Applied Physics, Czech Technical University, Prague, Czech Republic.
Jozef RosinaCzech Technical University in Prague, Faculty of Biomedical Engineering, Kladno, Czech Republic.
Petr ZachSpecialized Laboratory of Experimental Imaging Third Faculty of Medicine, Charles University, Institute of Experimental and Applied Physics and Faculty of Biomedical Engineering, Czech Technical University in Prague, Prague, Czech Republic.
Charles University · CZCzech Technical University in Prague · CZUniversity of Florence · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Micro-CT imaging is a well-established morphological method for the visualization of animal models. We used ethanol fixation of the mouse brains to perform high-resolution micro-CT scans showing in great details brain grey and white matters. It was possible to identify more than 50 neuroanatomical structures on the 5 selected coronal sections. Among white matter structures, we identified fornix, medial lemniscus, crossed tectospinal pathway, mammillothalamic tract, and the sensory root of the trigeminal ganglion. Among grey matter structures, we identified basal nuclei, habenular complex, thalamic nuclei, amygdala, subparts of hippocampal formation, superior colliculi, Edinger-Westphal nucleus, and others. We suggest that micro-CT of the mouse brain could be used for neurohistological lesions evaluation as an alternative to classical neurohistology because it does not destroy brain tissue.

Indexed as

Tissue FixationX-Ray MicrotomographyAnimalsBrainEthanolMaleMiceEthanol

Identifiers

PMID31392208
PMCPMC6662504
OpenAlexW2960642900

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.