Evidence map›Paper›PMID 31391571›Full record

ArticleScientific reports2019

An Effective Electric Dipole Model for Voltage-induced Gating Mechanism of Lysenin.

Radwan Al Faouri, Eric Krueger, Vivek Govind Kumar, Daniel Fologea, David Straub, Hanan Alismail, Qusay Alfaori, Alicia Kight, Jess Ray, Ralph Henry and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Towards the Idea of Molecular Brains.International journal of molecular sciences · 2021
    Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 2 countries.

Radwan Al FaouriDivision of Sciences and Mathematics, University of the Ozarks, Clarksville, AR, 72830, USA. radwanaf@uark.edu.ORCID http://orcid.org/0000-0003-0780-9104
Eric KruegerDepartment of Physics, Boise State University, Boise, ID, 83725, USA.
Vivek Govind KumarDepartment of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR, 72701, USA.
Daniel FologeaDepartment of Physics, Boise State University, Boise, ID, 83725, USA.
David StraubDepartment of Biochemistry and Molecular Biology, School of Medicine, University of Arkansas for Medical Sciences, Little Rock, AR, 72205, USA.
Hanan AlismailKing Abdullah International Medical Research Center, Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia.
Qusay AlfaoriDepartment of Biomedical Engineering, University of Arkansas, Fayetteville, AR, 72701, USA.
Alicia KightDepartment of Biological Sciences, University of Arkansas, Fayetteville, AR, 72701, USA.
Jess RayDepartment of Physics, University of Arkansas, Fayetteville, AR, 72701, USA.
Ralph HenryDepartment of Biological Sciences, University of Arkansas, Fayetteville, AR, 72701, USA.
Mahmoud MoradiDepartment of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR, 72701, USA.ORCID http://orcid.org/0000-0002-0601-402X
Gregory SalamoDepartment of Physics, University of Arkansas, Fayetteville, AR, 72701, USA.
University of Arkansas at Fayetteville · USBoise State University · USKing Saud University · SAUniversity of Arkansas for Medical Sciences · USUniversity of the Ozarks · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lysenin is a pore-forming toxin, which self-inserts open channels into sphingomyelin containing membranes and is known to be voltage regulated. The mechanistic details of its voltage gating mechanism, however, remains elusive despite much recent efforts. Here, we have employed a novel combination of experimental and computational techniques to examine a model for voltage gating, that is based on the existence of an "effective electric dipole" inspired by recent reported structures of lysenin. We support this mechanism by the observations that (i) the charge-reversal and neutralization substitutions in lysenin result in changing its electrical gating properties by modifying the strength of the dipole, and (ii) an increase in the viscosity of the solvent increases the drag force and slows down the gating. In addition, our molecular dynamics (MD) simulations of membrane-embedded lysenin provide a mechanistic picture for lysenin conformational changes, which reveals, for the first time, the existence of a lipid-dependent bulge region in the pore-forming module of lysenin, which may explain the gating mechanism of lysenin at a molecular level.

Indexed as

Lipid MetabolismMolecular Dynamics SimulationCholesterolIon Channel GatingLipidsMutationPhosphatidylcholinesProtein DomainsProtein EngineeringRecombinant ProteinsSphingomyelinsToxins, BiologicalasolectinCholesterolLipidslyseninPhosphatidylcholinesRecombinant ProteinsSphingomyelinsToxins, Biological

Identifiers

PMID31391571
PMCPMC6686002
OpenAlexW2964643462

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.