ArticleScientific reports2019
Ensilication Improves the Thermal Stability of the Tuberculosis Antigen Ag85b and an Sbi-Ag85b Vaccine Conjugate.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 33 citations in OpenAlex.
- Article
- Thermal Stabilisation of Lysozyme through Ensilication.Molecules (Basel, Switzerland) · 2024Article
- Diagnostics of Tuberculosis with Single-Walled Carbon Nanotube-Based Field-Effect Transistors.ACS sensors · 2024Article
- Clinical and Preclinical Methods of Heat-Stabilization of Human Vaccines.Molecular pharmaceutics · 2024Review
- DeepTP: A Deep Learning Model for Thermophilic Protein Prediction.International journal of molecular sciences · 2023Article
- Lyophilization Process Engineering and Thermostability of ID93 + GLA-SE, a Single-Vial Adjuvanted Subunit Tuberculosis Vaccine Candidate for Use in Clinical Studies.Frontiers in drug delivery · 2022Article
- A Modular Biomaterial Scaffold-Based Vaccine Elicits Durable Adaptive Immunity to Subunit SARS-CoV-2 Antigens.Advanced healthcare materials · 2021Article
- Article
- Development of thermostable vaccine adjuvants.Expert review of vaccines · 2021Review
- Non-viral COVID-19 vaccine delivery systems.Advanced drug delivery reviews · 2021Review
- Thermal resilience of ensilicated lysozymeRSC advances · 2020Article
- Bluetongue virus viral protein 7 stability in the presence of glycerol and sodium chloride.Clinical and experimental vaccine research · 2020Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
There is an urgent need for the development of vaccine thermostabilisation methodologies as the maintenance of a continuous and reliable cold chain remains a major hurdle to the global distribution of safe and effective vaccines. Ensilication, a method that encases proteins in a resistant silica cage has been shown to physically prevent the thermal denaturation of a number of model proteins. In this study we investigate the utility of this promising approach in improving the thermal stability of antigens and vaccine conjugates highly relevant to the development of candidate tuberculosis vaccines, including antigen 85b conjugated with the Staphylococcus aureus-protein based adjuvant Sbi. Here we analyse the sensitivity of these constructs to thermal denaturation and demonstrate for the first time the benefits of ensilication in conferring these vaccine-relevant proteins with protection against temperature-induced loss of structure and function without the need for refrigeration. Our results reveal the potential of ensilication in facilitating the storage and transport of vaccines at ambient temperatures in the future and therefore in delivering life-saving vaccines globally, and in particular to remote areas of developing countries where disease rates are often highest.
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Registered trials
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