ArticleStem cell research & therapy2019
Establishment of human trophoblast stem cells from human induced pluripotent stem cell-derived cystic cells under micromesh culture.
Article in Stem cell research & therapy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 53 citations in OpenAlex.
- Patient-specific trophoblast stem cells for placental medicine.Trends in molecular medicine · 2026Review
- Advancing Human Placental Modeling Through Stem-Cell-Derived Trophoblast Organoids and Reprogramming Innovations.Biomedicines · 2026Review
- BMP4 and FGF2 promote heterogeneous priming including extraembryonic lineages in porcine expanded potential stem cells.Frontiers in cell and developmental biology · 2026Article
- The modeling of human implantation and early placentation: achievements and perspectives.Human reproduction update · 2025Review
- The role of placenta Hofbauer cells during pregnancy and pregnancy complications.Obstetrics & gynecology science · 2025Article
- Generation of Bona Fide Human Induced Trophoblast Stem Cells by Direct Reprogramming of Term Umbilical Cord Cells.International journal of molecular sciences · 2024Article
- Rapid retinoic acid-induced trophoblast cell model from human induced pluripotent stem cells.Scientific reports · 2024Article
- Trophoblast stem cell-based organoid models of the human placental barrier.Nature communications · 2024Article
- A comprehensive review of human trophoblast fusion models: recent developments and challenges.Cell death discovery · 2023Review
- Current Strategies of Modeling Human Trophoblast Using Human Pluripotent Stem Cells in vitro.Current protocols · 2023Article
- Article
- Pluripotency-independent induction of human trophoblast stem cells from fibroblasts.Nature communications · 2023Article
- Neurotrophin-4 promotes the specification of trophectoderm lineage after parthenogenetic activation and enhances porcine early embryonic development.Frontiers in cell and developmental biology · 2023Article
- Exploring maternal-fetal interface with in vitro placental and trophoblastic models.Frontiers in cell and developmental biology · 2023Review
- Establishment of human induced trophoblast stem cells via reprogramming of fibroblasts.Nature protocols · 2022Review
- Accessing the human trophoblast stem cell state from pluripotent and somatic cells.Cellular and molecular life sciences : CMLS · 2022Review
- Single-cell analysis of embryoids reveals lineage diversification roadmaps of early human development.Cell stem cell · 2022Article
- The role of BMP4 signaling in trophoblast emergence from pluripotency.Cellular and molecular life sciences : CMLS · 2022Review
- How trophoblasts fuse: an in-depth look into placental syncytiotrophoblast formation.Cellular and molecular life sciences : CMLS · 2022Review
- Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTrophoblasts as a specific cell lineage are crucial for the correct function of the placenta. Human trophoblast stem cells (hTSCs) are a proliferative population that can differentiate into syncytiotrophoblasts and extravillous cytotrophoblasts. Many studies have reported that chemical supplements induce the differentiation of trophoblasts from human induced pluripotent stem cells (hiPSCs). However, there have been no reports of the establishment of proliferative hTSCs from hiPSCs. Our previous report showed that culturing hiPSCs on micromesh as a bioscaffold induced cystic cells with trophoblast properties. Here, we aimed to establish hTSCs from hiPSCs.
methodsWe used the micromesh culture technique to induce hiPSC differentiation into trophoblast cysts. We then reseeded and purified cystic cells.
resultsThe cells derived from the reseeded cysts were highly proliferative. Low expression levels of pluripotency genes and high expression levels of TSC-specific genes were detected in proliferative cells. The cells could be passaged, and further directional differentiation into syncytiotrophoblast- and extravillous cytotrophoblast-like cells was confirmed by marker expression and hormone secretion.
conclusionsWe established hiPSC-derived hTSCs, which may be applicable for studying the functions of trophoblasts and the placenta. Our experimental system may provide useful tools for understanding the pathogenesis of infertility owing to trophoblast defects in the future.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.