ArticlePLoS neglected tropical diseases2019
An improved genome assembly of the fluke Schistosoma japonicum.
Article in PLoS neglected tropical diseases, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed, 91 citations in OpenAlex.
- NResearch (Washington, D.C.) · 2026Article
- Subtelomeric plasticity contributes to gene family expansion in the human parasitic flatworm Schistosoma mansoni.BMC genomics · 2024Article
- Targeting SmCB1: Perspectives and Insights to Design Antischistosomal Drugs.Current medicinal chemistry · 2024Review
- Trematode Genomics and Proteomics.Advances in experimental medicine and biology · 2024Article
- Chromosome-scale genome of the human blood fluke Schistosoma mekongi and its implications for public health.Infectious diseases of poverty · 2023Article
- Lentiviral Transduction-based CRISPR/Cas9 Editing ofCurrent genomics · 2023Article
- Construction and characterization of microsatellite markers for the Schistosoma japonicum isolate from a hilly area of China based on whole genome sequencing.Parasitology research · 2023Article
- Phylogenomic Analysis of 155 Helminth Species Reveals Widespread Absence of Oxygen Metabolic Capacity.Genome biology and evolution · 2023Article
- What about the Cytoskeletal and Related Proteins of Tapeworms in the Host's Immune Response? An Integrative Overview.Pathogens (Basel, Switzerland) · 2023Review
- Genetic difference between two Schistosoma japonicum isolates with contrasting cercarial shedding patterns revealed by whole genome sequencing.Parasite (Paris, France) · 2023Article
- Integrating genomic and epidemiologic data to accelerate progress toward schistosomiasis elimination.eLife · 2022Review
- Genome-wide analysis of Schistosoma mansoni reveals limited population structure and possible praziquantel drug selection pressure within Ugandan hot-spot communities.PLoS neglected tropical diseases · 2022Article
- Experimental Schistosoma japonicum-induced pulmonary hypertension.PLoS neglected tropical diseases · 2022Article
- Chromosome-level genome of Schistosoma haematobium underpins genome-wide explorations of molecular variation.PLoS pathogens · 2022Article
- Prospects for genomic surveillance for selection in schistosome parasites.Frontiers in epidemiology · 2022Review
- Article
- CRISPR interference for sequence-specific regulation of fibroblast growth factor receptor A inFrontiers in immunology · 2022Article
- Synergistic effect of combination chemotherapy with praziquantel and DW-3-15 for Schistosoma japonicum in vitro and in vivo.Parasites & vectors · 2021Article
- The parasite Schistocephalus solidus secretes proteins with putative host manipulation functions.Parasites & vectors · 2021Article
- Review
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Authors and funding
15 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSchistosoma japonicum is a parasitic flatworm that causes human schistosomiasis, which is a significant cause of morbidity in China and the Philippines. A single draft genome was available for S. japonicum, yet this assembly is very fragmented and only covers 90% of the genome, which make it difficult to be applied as a reference in functional genome analysis and genes discovery.
findingsIn this study, we present a high-quality assembly of the fluke S. japonicum genome by combining 20 G (~53X) long single molecule real time sequencing reads with 80 G (~ 213X) Illumina paired-end reads. This improved genome assembly is approximately 370.5 Mb, with contig and scaffold N50 length of 871.9 kb and 1.09 Mb, representing 142.4-fold and 6.2-fold improvement over the released WGS-based assembly, respectively. Additionally, our assembly captured 85.2% complete and 4.6% partial eukaryotic Benchmarking Universal Single-Copy Orthologs. Repetitive elements account for 46.80% of the genome, and 10,089 of the protein-coding genes were predicted from the improved genome, of which 96.5% have been functionally annotated. Lastly, using the improved assembly, we identified 20 significantly expanded gene families in S. japonicum, and those genes were primarily enriched in functions of proteolysis and protein glycosylation.
conclusionsUsing the combination of PacBio and Illumina Sequencing technologies, we provided an improved high-quality genome of S. japonicum. This improved genome assembly, as well as the annotation, will be useful for the comparative genomics of the flukes and more importantly facilitate the molecular studies of this important parasite in the future.
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