ArticlePlant methods2019
A customizable method to characterize
Article in Plant methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 19 citations in OpenAlex.
- The interplay between virus infection and water-related stress is mediated by the plant metabolism of ascorbic acid.The New phytologist · 2025Article
- Functional Relationships of Two NFU Proteins in Maintaining the Abundances of Mitochondrial Iron-Sulfur Proteins.Plant direct · 2025Article
- Evaluating Plant Drought Resistance with a Raspberry Pi and Time-lapse Photography.Bio-protocol · 2023Article
- Uncoupling differential water usage from drought resistance in a dwarf Arabidopsis mutant.Plant physiology · 2022Article
- Non-Photochemical Quenching under Drought and Fluctuating Light.International journal of molecular sciences · 2022Article
- Ectopic expression of GmHP08 enhances resistance of transgenic Arabidopsis toward drought stress.Plant cell reports · 2021Article
- Phenotyping in Arabidopsis and Crops-Are We Addressing the Same Traits? A Case Study in Tomato.Genes · 2020Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCharacterization of the dynamic response of plant transpiration to decreasing soil water content in a reproducible way is required for the correct phenotyping of traits related to water saving strategies. Nowadays, an increasing number of automated high throughput platforms are available, but their development requires a great economic investment and it is not always desirable/feasible to outsource these analyses. We propose a medium-throughput protocol to characterize transpiration responses to decreasing soil moisture in a quantitative and highly reproducible way with a minimum investment of resources.
resultsThe quantitative characterization of plant responses to a decreasing soil water content using our phenotyping platform has showed high reproducibility between different experiments. The proposed irrigation strategy allowed us to harvest plants ranging from a well-watered condition to the loss-of-turgor point in a predictable and controlled way. Coupling this protocol with hormone profiling allows investigation of hormonal responses (metabolite accumulation as well as plant sensitivity) to water stress. As a proof-of-concept, we have characterized the dynamic responses of leaf transpiration to decreasing soil water contents in an abscisic acid (ABA) deficient genotype (
conclusionsThis protocol allows for assessment of quantitative differences in rosette transpiration responses to water depletion in both ABA biosynthesis mutants and genotypes with altered sensitivity to the hormone. Data indicate a correlation between ABA levels and/or hormone perception and growth rate and/or water content. The protocol guarantees the correct application of water stress to adult plants, which is essential to understand responses of mutants and/or natural accessions.
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