Evidence map›Paper›PMID 31386303›Full record

ArticleJournal of cellular and molecular medicine2019

Molecular mechanism of tumour necrosis factor alpha regulates hypocretin (orexin) expression, sleep and behaviour.

Shuqin Zhan, Pulin Che, Xue-Ke Zhao, Ning Li, Yan Ding, Jianghong Liu, Spring Li, Karyn Ding, Lynn Han, Zhaoyang Huang and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cellular and molecular medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed, 2 pooled it
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 2 syntheses or guidelines pooled it, 20 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 2 institutions in 2 countries.

Shuqin ZhanDepartment of Neurology, Xuanwu Hospital, Capital Medical University, Beijing, China.ORCID 0000-0001-6134-4358
Pulin CheDepartment of Medicine, University of Alabama at Birmingham, Birmingham, AL, USA.
Xue-Ke ZhaoDepartment of Medicine, University of Alabama at Birmingham, Birmingham, AL, USA.
Ning LiDepartment of Neurology, Xuanwu Hospital, Capital Medical University, Beijing, China.
Yan DingDepartment of Neurology, Xuanwu Hospital, Capital Medical University, Beijing, China.
Jianghong LiuDepartment of Neurology, Xuanwu Hospital, Capital Medical University, Beijing, China.
Spring LiDepartment of Medicine, University of Alabama at Birmingham, Birmingham, AL, USA.
Karyn DingDepartment of Medicine, University of Alabama at Birmingham, Birmingham, AL, USA.
Lynn HanDepartment of Medicine, University of Alabama at Birmingham, Birmingham, AL, USA.
Zhaoyang HuangDepartment of Neurology, Xuanwu Hospital, Capital Medical University, Beijing, China.
Liyong WuDepartment of Neurology, Xuanwu Hospital, Capital Medical University, Beijing, China.
Yuping WangDepartment of Neurology, Xuanwu Hospital, Capital Medical University, Beijing, China.
Meng HuDepartment of Neurology, Xuanwu Hospital, Capital Medical University, Beijing, China.
Xiaosi HanNeurology, University of Alabama at Birmingham, Birmingham, AL, USA.
Qiang DingDepartment of Medicine, University of Alabama at Birmingham, Birmingham, AL, USA.
Capital Medical University · CNUniversity of Alabama at Birmingham · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Hypocretin 1 and hypocretin 2 (orexin A and B) regulate sleep, wakefulness and emotion. Tumour necrosis factor alpha (TNF-α) is an important neuroinflammation mediator. Here, we examined the effects of TNF-α treatment on hypocretin expression in vivo and behaviour in mice. TNF-α decreased hypocretin 1 and hypocretin 2 expression in a dose-dependent manner in cultured hypothalamic neurons. TNF-α decreased mRNA stability of prepro-hypocretin, the single precursor of hypocretin 1 and hypocretin 2. Mice challenged with TNF-α demonstrated decreased expression of prepro-hypocretin, hypocretin 1 and hypocretin 2 in hypothalamus. In response to TNF-α, prepro-hypocretin mRNA decay was increased in hypothalamus. TNF-α neutralizing antibody restored the expression of prepro-hypocretin, hypocretin 1 and hypocretin 2 in vivo in TNF-α challenged mice, supporting hypocretin system can be impaired by increased TNF-α through decreasing hypocretin expression. Repeated TNF-α challenge induced muscle activity during rapid eye movement sleep and sleep fragmentation, but decreased learning, cognition and memory in mice. TNF-α neutralizing antibody blocked the effects of TNF-α; in contrast, hypocretin receptor antagonist enhanced the effects of TNF-α. The data support that TNF-α is involved in the regulation of hypocretin expression, sleep and cognition. The findings shed some lights on the role of neuroinflammation in neurodegenerative diseases including Alzheimer's disease and Parkinson's disease.

Indexed as

Behavior, AnimalSleepAnimalsAntibodies, NeutralizingCells, CulturedCognitionGene Expression RegulationHypothalamusMemoryMice, Inbred C57BLMusclesNeuronsOrexinsRNA, MessengerRNA StabilitySignal TransductionAntibodies, NeutralizingHcrt protein, mouseOrexinsRNA, MessengerTumor Necrosis Factor-alphaAlzheimerhypocretinorexinParkinsonrapid eye movement sleep behaviour disorderTNF

Identifiers

PMID31386303
PMCPMC6787512
OpenAlexW2965715089

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.