ArticleJournal of biomedical optics2019
Propagating-path uniformly scanned light sheet excitation microscopy for isotropic volumetric imaging of large specimens.
Article in Journal of biomedical optics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Axially swept open-top light-sheet microscopy for densely labeled clinical specimens.Optics letters · 2024Article
- High-resolution open-top axially swept light sheet microscopy.BMC biology · 2023Article
- High contrast, isotropic, and uniform 3D-imaging of centimeter-scale scattering samples using structured illumination light-sheet microscopy with axial sweeping.Biomedical optics express · 2022Article
- Open-top axially swept light-sheet microscopy.Biomedical optics express · 2021Article
- High-speed axially swept light sheet microscopy using a linear MEMS phased array for isotropic resolution.Journal of biomedical optics · 2020Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We demonstrate a propagating-path uniformly scanned light sheet excitation (PULSE) microscopy based on the oscillation of voice coil motor that can rapidly drive a thin light sheet along its propagation direction. By synchronizing the rolling shutter of a camera with the motion of laser sheet, we can obtain a uniform plane-illuminated image far beyond the confocal range of Gaussian beam. A stable 1.7-μm optical sectioning under a 3.3 mm × 3.3 mm wide field of view (FOV) has been achieved for up to 20 Hz volumetric imaging of large biological specimens. PULSE method transforms the extent of plane illumination from one intrinsically limited by the short confocal range (μm scale) to one defined by the motor oscillation range (mm scale). Compared to the conventional Gaussian light sheet imaging, our method greatly mitigates the compromise of axial resolution and successfully extends the FOV over 100 times. We demonstrate the applications of PULSE method by rapidly imaging cleared mouse spinal cord and live zebrafish larva at isotropic subcellular resolution.
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Registered trials
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