Evidence map›Paper›PMID 31385277›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2019

Forward and Reverse Genetic Analysis of Chlamydia.

Mateusz Kędzior, Robert J Bastidas

Open access · greenAbstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.9field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
  2. Article
  3. Editorial:Frontiers in cellular and infection microbiology · 2024
    Article
  4. Frontiers in cell and developmental biology · 2024
    Review
  5. Advances in genetic manipulation ofFrontiers in immunology · 2023
    Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Mateusz KędziorDepartment of Molecular Genetics and Microbiology, Duke University, Durham, NC, USA.
Robert J BastidasDepartment of Molecular Genetics and Microbiology, Duke University, Durham, NC, USA. robert.bastidas@duke.edu.
Duke University · US

Funding

Posttranslational modifications of host proteins by Chlamydia effectorsR21AI140019 · NIAID · DUKE UNIVERSITY · PI BASTIDAS, ROBERT J · 2018 to 2019
$441k
NIAID NIH HHS R21 AI140019
6 · The paper itself

Abstract

Chlamydia is a major etiological agent of human disease that affects millions of individuals worldwide. Historically, our understanding of the mechanisms that contribute to its pathogenesis has been limited. However, the recent development of powerful genetic tools for manipulating Chlamydia has resulted in significant gains in our ability to dissect its virulence mechanisms. These tools have overcome several barriers for manipulating intracellular pathogens and are amenable for the routine genetic engineering of Chlamydia. Here, we provide several detailed protocols for performing genetic analysis in Chlamydia trachomatis allowing investigators to elucidate how this obligate intracellular pathogen causes disease.

Indexed as

AnimalsChlamydia InfectionsChlamydia trachomatisChlorocebus aethiopsDNA, BacterialGenetic LinkageHumansMutagenesisPolymorphism, Single NucleotideReverse GeneticsVero CellsDNA, BacterialChlamydia chemical mutagenesisChlamydia DNA isolationChlamydia geneticsChlamydia linkage analysisChlamydia plaque assayChlamydia plasmid transformation

Identifiers

PMID31385277
PMCPMC7021217
OpenAlexW2966521879

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.