ArticleScientific reports2019
Small RNA profiling in Pinus pinaster reveals the transcriptome of developing seeds and highlights differences between zygotic and somatic embryos.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 37 citations in OpenAlex.
- New molecular players: siRNA expression and gene regulation in Hordeum vulgare L. ageing seeds after germination.BMC genomics · 2026Article
- Temperature-induced variation in the transcriptome of maritime pine (Pinus pinaster Ait.) embryogenic masses modulates the phenotype of the derived plants.BMC genomics · 2025Article
- Article
- MicroRNA-mediated post-transcriptional regulation of Pinus pinaster response and resistance to pinewood nematode.Scientific reports · 2022Article
- miR172 RegulatesCells · 2022Article
- Spatiotemporal expression profile of novel and known small RNAs throughout rice plant development focussing on seed tissues.BMC genomics · 2022Article
- miR160 InteractsFrontiers in plant science · 2022Article
- Regulatory non-coding RNAs: Emerging roles during plant cell reprogramming andFrontiers in plant science · 2022Review
- Article
- Review
- Small Non-Coding RNAs at the Crossroads of Regulatory Pathways Controlling Somatic Embryogenesis in Seed Plants.Plants (Basel, Switzerland) · 2021Review
- Transcriptomic Time-Series Analyses of Gene Expression Profile During Zygotic Embryo Development inFrontiers in genetics · 2021Article
- Research Tools for the Functional Genomics of Plant miRNAs During Zygotic and Somatic Embryogenesis.International journal of molecular sciences · 2020Review
- Epigenetic Regulation of Auxin-Induced Somatic Embryogenesis in Plants.International journal of molecular sciences · 2020Review
- Current Perspectives on the Auxin-Mediated Genetic Network that Controls the Induction of Somatic Embryogenesis in Plants.International journal of molecular sciences · 2020Review
- miRNA expression profiling and zeatin dynamic changes in a new model system of in vivo indirect regeneration of tomato.PloS one · 2020Article
- Tissue-specific transposon-associated small RNAs in the gymnosperm tree, Norway spruce.BMC genomics · 2019Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Regulation of seed development by small non-coding RNAs (sRNAs) is an important mechanism controlling a crucial phase of the life cycle of seed plants. In this work, sRNAs from seed tissues (zygotic embryos and megagametophytes) and from somatic embryos of Pinus pinaster were analysed to identify putative regulators of seed/embryo development in conifers. In total, sixteen sRNA libraries covering several developmental stages were sequenced. We show that embryos and megagametophytes express a large population of 21-nt sRNAs and that substantial amounts of 24-nt sRNAs were also detected, especially in somatic embryos. A total of 215 conserved miRNAs, one third of which are conifer-specific, and 212 high-confidence novel miRNAs were annotated. MIR159, MIR171 and MIR394 families were found in embryos, but were greatly reduced in megagametophytes. Other families, like MIR397 and MIR408, predominated in somatic embryos and megagametophytes, suggesting their expression in somatic embryos is associated with in vitro conditions. Analysis of the predicted miRNA targets suggests that miRNA functions are relevant in several processes including transporter activity at the cotyledon-forming stage, and sulfur metabolism across several developmental stages. An important resource for studying conifer embryogenesis is made available here, which may also provide insightful clues for improving clonal propagation via somatic embryogenesis.
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