ArticleThe Journal of cell biology1988
Lateral segregation of neutrophil chemotactic receptors into actin- and fodrin-rich plasma membrane microdomains depleted in guanyl nucleotide regulatory proteins.
Article in The Journal of cell biology, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 102 citations in OpenAlex.
- Actin dynamics rapidly reset chemoattractant receptor sensitivity following adaptation in neutrophils.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Article
- Upregulation of vitamin D binding protein (Gc-globulin) binding sites during neutrophil activation from a latent reservoir in azurophil granules.Molecular immunology · 2007Article
- Sequential degradation of alphaII and betaII spectrin by calpain in glutamate or maitotoxin-stimulated cells.Biochemistry · 2007Article
- Plasma membrane organization is essential for balancing competing pseudopod- and uropod-promoting signals during neutrophil polarization and migration.Molecular biology of the cell · 2005Article
- Preparation of secretory vesicle-free plasma membranes by isopycnic sucrose gradient fractionation of neutrophils purified by the gelatin method.Cytotechnology · 2004Article
- Dynamic distribution of chemoattractant receptors in living cells during chemotaxis and persistent stimulation.The Journal of cell biology · 1997Article
- Escherichia coli-induced activation of neutrophil NADPH-oxidase: lipopolysaccharide and formylated peptides act synergistically to induce release of reactive oxygen metabolites.Infection and immunity · 1995Article
- Insulation of a G protein-coupled receptor on the plasmalemmal surface of the pancreatic acinar cell.The Journal of cell biology · 1995Article
- Chemoattractant-induced NADPH oxidase activity in human monocytes is terminated without any association of receptor-ligand complex to cytoskeleton.Inflammation · 1995Article
- Specific inhibition of leukotriene B4 (LTB4)-induced neutrophil emigration by 20-hydroxy LTB4: implications for the regulation of inflammatory responses.British journal of pharmacology · 1993Article
- Stimulus-induced dissociation of alpha subunits of heterotrimeric GTP-binding proteins from the cytoskeleton of human neutrophils.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Stimulus-response uncoupling in the neutrophil. Adenosine A2-receptor occupancy inhibits the sustained, but not the early, events of stimulus transduction in human neutrophils by a mechanism independent of actin-filament formation.The Biochemical journal · 1992Article
- Down-regulation of receptor antigen in leukotriene B4-induced chemotactic deactivation of human polymorphonuclear leucocytes.Immunology · 1991Article
- Three different actin filament assemblies occur in every hair cell: each contains a specific actin crosslinking protein.The Journal of cell biology · 1991Article
- The adenosine/neutrophil paradox resolved: human neutrophils possess both A1 and A2 receptors that promote chemotaxis and inhibit O2 generation, respectively.The Journal of clinical investigation · 1990Article
- Calmodulin and calcium-dependent protease I coordinately regulate the interaction of fodrin with actin.Proceedings of the National Academy of Sciences of the United States of America · 1990Article
- Subcellular distribution of alpha 2-adrenergic receptors, pertussis-toxin substrate and adenylate cyclase in human platelets.The Biochemical journal · 1990Article
- Fodrin as a differentiation marker. Redistributions in colonic neoplasia.The American journal of pathology · 1989Article
- Studies on the uptake, binding and metabolism of leukotriene B4 by human neutrophils.Immunology · 1989Article
- Association of ligand-receptor complexes with actin filaments in human neutrophils: a possible regulatory role for a G-protein.The Journal of cell biology · 1989Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Subcellular fractions were prepared from human neutrophils desensitized at 15 degrees C with stimulatory doses of the photoaffinity derivative F-Met-Leu-Phe-N epsilon-(2-(rho-azido[125I]salicylamido)ethyl-1,3'- dithio-propionyl)-Lys. The covalently labeled receptors were found in a membrane fraction of higher density than those from cells preexposed to ligand at 4 degrees C but not desensitized. The denser fraction (rho approximately equal to 1.155 g/cc) was the cellular locus of the membrane associated cytoskeletal proteins, actin, and fodrin, as detected immunologically on western blots. The light fraction (rho approximately equal to 1.135), cosedimented with neutrophil plasma membrane markers, plasma membrane guanyl nucleotide regulatory proteins, and several characteristic polypeptides identified by SDS-PAGE, including a major 72-kD species. The photoaffinity-labeled species in either case showed the same mobility on SDS-PAGE (Mr = 50,000-70,000) corresponding to previously reported values for N-formyl chemotactic receptors. These labeled receptors were sensitive to proteolysis after exposure of the intact photoaffinity-labeled cells to papain at 4 degrees C. We conclude that (a) the fractions isolated are probably derived from different lateral microdomains of the surface of human neutrophils; (b) the higher density fraction contains occupied N-formyl-chemotactic receptors previously shown to have been converted, to a high affinity, slowly dissociating form coisolating with neutrophil cytoskeleton and implicated in the termination of formyl peptide-induced neutrophil activation; and (c) the translocation of receptors to these microdomains may serve to compartmentalize receptors and perhaps regulate the interaction of the receptor/G-protein transduction pair.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.