ReviewMolecules (Basel, Switzerland)2019
Real-Time 3D Single Particle Tracking: Towards Active Feedback Single Molecule Spectroscopy in Live Cells.
Review in Molecules (Basel, Switzerland), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed.
- Mapping Nanoscale Forces and Potentials in Live Cells with Microsecond 3D Single-Particle Tracking.Chemical & biomedical imaging · 2026Article
- Review of Machine Learning for Single-Particle Tracking: Methods, Challenges, and Biophysical Insights.Chemical & biomedical imaging · 2026Review
- 3D Single-Virus Tracking: Advances in Methodology and Labeling Strategies Towards Probing the Virus-Epithelium Interaction.Viruses · 2026Review
- High-speed 3D single-virus tracking reveals actin-aided viral trafficking of SARS-CoV-2 on the plasma membrane.bioRxiv : the preprint server for biology · 2026Article
- Super-Resolving Particle Diffusion Heterogeneity in Porous Hydrogels via High-Speed 3D Active-Feedback Single-Particle Tracking Microscopy.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Elementary Charge Characterization of Single Quantum Dots in Solution.Nano letters · 2025Article
- Super-resolving particle diffusion heterogeneity in porous hydrogels via high-speed 3D active-feedback single-particle tracking microscopy.bioRxiv : the preprint server for biology · 2025Article
- Long axial range 3D single-particle tracking using birefringent substrates.Nature communications · 2025Article
- Three-dimensional random-access confocal microscopy with 3D remote focusing system.Communications engineering · 2024Article
- Single-nanoparticle electrophoretic mobility determination and trapping using active-feedback 3D tracking.bioRxiv : the preprint server for biology · 2024Article
- Unravelling the Mystery inside Cells by Using Single-Molecule Fluorescence Imaging.Journal of imaging · 2023Review
- Active-Feedback 3D Single-Molecule Tracking Using a Fast-Responding Galvo Scanning Mirror.The journal of physical chemistry. A · 2023Article
- Information optimization of laser scanning microscopes for real-time feedback-driven single particle tracking.Optics express · 2023Article
- Recent Advances in Single-Molecule Tracking and Imaging Techniques.Annual review of analytical chemistry (Palo Alto, Calif.) · 2023Review
- Study liquid-liquid phase separation with optical microscopy: A methodology review.APL bioengineering · 2023Review
- Fluorescence lifetime tracking and imaging of single moving particles assisted by a low-photon-count analysis algorithm.Biomedical optics express · 2023Article
- Single-molecule imaging in the primary cilium.Methods in cell biology · 2023Article
- Single-molecule tracking for studying protein dynamics and target-search mechanism in live cells of S. cerevisiae.STAR protocols · 2022Article
- Impact ofInternational journal of molecular sciences · 2022Review
- Capturing the start point of the virus-cell interaction with high-speed 3D single-virus tracking.Nature methods · 2022Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Single molecule fluorescence spectroscopy has been largely implemented using methods which require tethering of molecules to a substrate in order to make high temporal resolution measurements. However, the act of tethering a molecule requires that the molecule be removed from its environment. This is especially perturbative when measuring biomolecules such as enzymes, which may rely on the non-equilibrium and crowded cellular environment for normal function. A method which may be able to un-tether single molecule fluorescence spectroscopy is real-time 3D single particle tracking (RT-3D-SPT). RT-3D-SPT uses active feedback to effectively lock-on to freely diffusing particles so they can be measured continuously with up to photon-limited temporal resolution over large axial ranges. This review gives an overview of the various active feedback 3D single particle tracking methods, highlighting specialized detection and excitation schemes which enable high-speed real-time tracking. Furthermore, the combination of these active feedback methods with simultaneous live-cell imaging is discussed. Finally, the successes in real-time 3D single molecule tracking (RT-3D-SMT) thus far and the roadmap going forward for this promising family of techniques are discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.