ArticleViruses2019
A Single Mutation N166D in Hemagglutinin Affects Antigenicity and Pathogenesis of H9N2 Avian Influenza Virus.
Article in Viruses, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- Article
- Genetic Evolution of H9N2 Avian Influenza Virus in Guangxi, China.Microorganisms · 2025Article
- Divergent Avian Influenza H10 Viruses from Sympatric Waterbird Species in Italy: Zoonotic Potential Assessment by Molecular Markers.Microorganisms · 2025Article
- Avian influenza overview December 2023-March 2024.EFSA journal. European Food Safety Authority · 2024Article
- Diversity of genotypes and pathogenicity of H9N2 avian influenza virus derived from wild bird and domestic poultry.Frontiers in microbiology · 2024Article
- Virus versus host: influenza A virus circumvents the immune responses.Frontiers in microbiology · 2024Review
- Avian influenza overview September-December 2023.EFSA journal. European Food Safety Authority · 2023Article
- Emergence of a new designated clade 16 with significant antigenic drift in hemagglutinin gene of H9N2 subtype avian influenza virus in eastern China.Emerging microbes & infections · 2023Article
- Antigenic mapping of the hemagglutinin of the H9 subtype influenza A viruses using sera from Japanese quail (Journal of virology · 2023Article
- Amino Acid Variation at Hemagglutinin Position 193 Impacts the Properties of H9N2 Avian Influenza Virus.Journal of virology · 2023Article
- Protection of SPF Chickens by H9N2 Y439 and G1 Lineage Vaccine against Homologous and Heterologous Viruses.Vaccines · 2023Article
- Identification of Hemagglutinin Mutations Caused by Neuraminidase Antibody Pressure.Microbiology spectrum · 2021Article
- Identification of key residues involved in the neuraminidase antigenic variation of H9N2 influenza virus.Emerging microbes & infections · 2021Article
- Molecular characteristics of the H9N2 avian influenza viruses in live poultry markets in Anhui Province, China, 2013 to 2018.Health science reports · 2021Article
- A Cross-Reactive Monoclonal Antibody Against Neuraminidases of Both H9N2 and H3N2 Influenza Viruses Shows Protection in Mice Challenging Models.Frontiers in microbiology · 2021Article
- Generation of a recombinant chickenized monoclonal antibody against the neuraminidase of H9N2 avian influenza virus.AMB Express · 2020Article
- H9 Influenza Viruses: An Emerging Challenge.Cold Spring Harbor perspectives in medicine · 2020Review
- Inventory of molecular markers affecting biological characteristics of avian influenza A viruses.Virus genes · 2019Review
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Some immune escape mutants of H9N2 virus and the corresponding mutations in hemagglutinin (HA) have been documented, but little is known about the impact of a single mutation on the antigenicity and pathogenesis of H9N2. In this study, seven critical sites in HA associated with the antigenicity were identified and the effects of a HA mutation (N166D) derived from a H9N2 escape mutant (m3F2) were investigated. Although N166D did not significantly affect viral replication in Madin-Darby canine kidney (MDCK) cells and viral shedding in the larynx and cloaca of chicken, N166D attenuated the pathogenesis of the virus in mice. Compared to the rescued RgPR8-H9_166D, RgPR8-H9_166N caused greater body weight loss and higher viral titers in the lungs of the infected mice. Moreover, hemagglutination inhibition (HI) assay for the sera from the chickens infected with wild type H9N2 and mutant m3F2 showed that N166D mutation could result in weak antibody response in chickens. Considering the field strains of H9N2 with N166D mutation are frequently isolated in the countries with H9N2 vaccination, the findings that the single mutation in HA, N166D, affected both the antigenicity and pathogenesis of H9N2 highlight the significance of surveillance on such mutation that may contribute to the failure of H9N2 vaccination in the field.
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