Evidence map›Paper›PMID 31381839›Full record

ArticleStem cells (Dayton, Ohio)2019

Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.

Vishal Menon, Ria Thomas, Claudio Elgueta, Marcus Horl, Teresia Osborn, Penny J Hallett, Marlene Bartos, Ole Isacson, Jan Pruszak

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells (Dayton, Ohio), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.4field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 16 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Article
  6. Neural Stem Cell Transplantation for Neurodegenerative Diseases.International journal of molecular sciences · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Vishal MenonDepartment of Molecular Embryology, Institute of Anatomy and Cell Biology, Faculty of Medicine, University of Freiburg, Freiburg, Germany.ORCID 0000-0002-3796-4781
Ria ThomasDepartment of Molecular Embryology, Institute of Anatomy and Cell Biology, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
Claudio ElguetaInstitute for Physiology I, Cellular and Systemic Neurophysiology, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
Marcus HorlDepartment of Molecular Embryology, Institute of Anatomy and Cell Biology, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
Teresia OsbornNeuroregeneration Laboratories, McLean Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Penny J HallettNeuroregeneration Laboratories, McLean Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Marlene BartosInstitute for Physiology I, Cellular and Systemic Neurophysiology, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
Ole IsacsonNeuroregeneration Laboratories, McLean Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Jan PruszakDepartment of Molecular Embryology, Institute of Anatomy and Cell Biology, Faculty of Medicine, University of Freiburg, Freiburg, Germany.ORCID 0000-0003-4297-4009
University of Freiburg · DEHarvard University · US

Funding

Wellcome Trust
6 · The paper itself

Abstract

Cell state-, developmental stage-, and lineage-specific combinatorial expression of cluster of differentiation (CD) molecules enables the identification of cellular subsets via multicolor flow cytometry. We describe an exhaustive characterization of neural cell types by surface antigens, exploiting human pluripotent stem cell-derived neural cell systems. Using multiwell screening approaches followed by detailed validation of expression patterns and dynamics, we exemplify a strategy for resolving cellular heterogeneity in stem cell paradigms. In addition to providing a catalog of surface antigens expressed in the neural lineage, we identified the transferrin receptor-1 (CD71) to be differentially expressed in neural stem cells and differentiated neurons. In this context, we describe a role for N-Myc proto-oncogene (MYCN) in maintaining CD71 expression in proliferating neural cells. We report that in vitro human stem cell-derived neurons lack CD71 surface expression and that the observed differential expression can be used to identify and enrich CD71

Indexed as

Antigens, CDAntigens, SurfaceBiomarkersCell DifferentiationFlow CytometryHumansNeuronsPluripotent Stem CellsProto-Oncogene MasReceptors, TransferrinAntigens, CDAntigens, SurfaceBiomarkersCD71 antigenMAS1 protein, humanProto-Oncogene MasReceptors, TransferrinCD49b (integrin α-2)CD71CD surface markersCell sortingCell therapyCell transplantationNeural cell selectionNeural stem cellsNeuronal differentiationPluripotent stem cellsTransferrin receptor

Identifiers

PMID31381839
PMCPMC6851846
OpenAlexW2964383005

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.