ArticlePLoS genetics2019
Methyl-CpG-binding domain 9 (MBD9) is required for H2A.Z incorporation into chromatin at a subset of H2A.Z-enriched regions in the Arabidopsis genome.
Article in PLoS genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 56 citations in OpenAlex.
- Article
- The chromatin reader MBD4 regulates temperature- and flowering-responsive transcription by recognizing histone arginine methylation.Plant communications · 2026Article
- MBD8 is required for LDL2-mediated transcriptional repression downstream of H3K9me2 in Arabidopsis.Nucleic acids research · 2026Article
- H3K4me2 orchestrates H2A.Z and Polycomb repressive marks in Arabidopsis.Nature communications · 2025Article
- Molecular mechanisms and biological functions of active DNA demethylation in plants.Epigenetics & chromatin · 2025Review
- Article
- Epigenetics in the modern era of crop improvements.Science China. Life sciences · 2025Review
- H3K4me3 binding ALFIN-LIKE proteins recruit SWR1 for gene-body deposition of H2A.Z.Genome biology · 2025Article
- Roles of methyl-CpG-binding-domain (MBD) protiens in regulation of biological processes in plants.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- The GTE4-EML chromatin reader complex concurrently recognizes histone acetylation and H3K4 trimethylation in Arabidopsis.The Plant cell · 2024Article
- Chromatin sensing: integration of environmental signals to reprogram plant development through chromatin regulators.Journal of experimental botany · 2024Review
- Proteomic profiling ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- Spotlight on Plant Bromodomain Proteins.Biology · 2023Review
- Contribution of the histone variant H2A.Z to expression of responsive genes in plants.Seminars in cell & developmental biology · 2023Review
- Advances in biological functions and mechanisms of histone variants in plants.Frontiers in genetics · 2023Review
- Histone Variants in the Specialization of Plant Chromatin.Annual review of plant biology · 2022Review
- Interactome of Arabidopsis Thaliana.Plants (Basel, Switzerland) · 2022Article
- COMPASS functions as a module of the INO80 chromatin remodeling complex to mediate histone H3K4 methylation in Arabidopsis.The Plant cell · 2021Article
- Spatiotemporal control of miR398 biogenesis, via chromatin remodeling and kinase signaling, ensures proper ovule development.The Plant cell · 2021Article
- Biological role and mechanism of chromatin readers in plants.Current opinion in plant biology · 2021Review
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4 authors at 1 institution in 1 country.
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Abstract
The SWR1 chromatin remodeling complex, which deposits the histone variant H2A.Z into nucleosomes, has been well characterized in yeast and animals, but its composition in plants has remained uncertain. We used the conserved SWR1 subunit ACTIN RELATED PROTEIN 6 (ARP6) as bait in tandem affinity purification experiments to isolate associated proteins from Arabidopsis thaliana. We identified all 11 subunits found in yeast SWR1 and the homologous mammalian SRCAP complexes, demonstrating that this complex is conserved in plants. We also identified several additional proteins not previously associated with SWR1, including Methyl-CpG-BINDING DOMAIN 9 (MBD9) and three members of the Alfin1-like protein family, all of which have been shown to bind modified histone tails. Since mbd9 mutant plants were phenotypically similar to arp6 mutants, we explored a potential role for MBD9 in H2A.Z deposition. We found that MBD9 is required for proper H2A.Z incorporation at thousands of discrete sites, which represent a subset of the genomic regions normally enriched with H2A.Z. We also discovered that MBD9 preferentially interacts with acetylated histone H4 peptides, as well as those carrying mono- or dimethylated H3 lysine 4, or dimethylated H3 arginine 2 or 8. Considering that MBD9-dependent H2A.Z sites show a distinct histone modification profile, we propose that MBD9 recognizes particular nucleosome modifications via its PHD- and Bromo-domains and thereby guides SWR1 to these sites for H2A.Z deposition. Our data establish the SWR1 complex as being conserved across eukaryotes and suggest that MBD9 may be involved in targeting the complex to specific genomic sites through nucleosomal interactions. The finding that MBD9 does not appear to be a core subunit of the Arabidopsis SWR1 complex, along with the synergistic phenotype of arp6;mbd9 double mutants, suggests that MBD9 also has important roles beyond H2A.Z deposition.
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