ArticleThe EMBO journal1988
Antibody engineering for the analysis of affinity maturation of an anti-hapten response.
Article in The EMBO journal, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 119 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
119 citing papers in PubMed, 289 citations in OpenAlex.
- Growing up as an Immunologist Among Molecular Geneticists.European journal of immunology · 2026Article
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- B Lymphocyte Protein Factories produced by Hematopoietic Stem Cell Gene Editing.bioRxiv : the preprint server for biology · 2026Article
- Antibody-mediated feedback modulates interclonal competition in the germinal center.bioRxiv : the preprint server for biology · 2025Article
- B lymphocytes that enter the germinal center late preferentially differentiate into memory cells that recognize subdominant epitopes.bioRxiv : the preprint server for biology · 2025Article
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- Autophagy is an upstream mediator of chromatin dynamics in normal and autoimmune germinal center B cells.The Journal of clinical investigation · 2025Article
- KLF2 expression in IgG plasma cells at their induction site regulates the migration program.The Journal of experimental medicine · 2025Article
- Article
- Lung B cells in ectopic germinal centers undergo affinity maturation.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Affinity Maturation for Antibody Engineering: The Critical Role of Residues on CDR Loops of Antibodies in Antigen Binding.Molecules (Basel, Switzerland) · 2025Article
- Affinity maturation of antibody responses is mediated by differential plasma cell proliferation.Science (New York, N.Y.) · 2025Article
- Article
- Affinity maturation of antibody responses is mediated by differential plasma cell proliferation.bioRxiv : the preprint server for biology · 2024Article
- PI3Kγ in B cells promotes antibody responses and generation of antibody-secreting cells.Nature immunology · 2024Article
- Csk restrains BCR-mediated ROS production and contributes to germinal center selection and affinity maturation.The Journal of experimental medicine · 2024Article
- Ion channel TRPV2 is critical in enhancing B cell activation and function.The Journal of experimental medicine · 2024Article
- Role of affinity in plasma cell development in the germinal center light zone.The Journal of experimental medicine · 2024Article
- STIM-mediated calcium influx regulates maintenance and selection of germinal center B cells.The Journal of experimental medicine · 2024Article
59 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The influence of structural variation, previously observed in a panel of V186.2 VH/V lambda 1-expressing anti-NP antibodies from the secondary response, on the affinity of these antibodies was examined by site-specific mutagenesis and recombinant antibody construction. A tryptophan----leucine exchange at position 33 in the VH segment of all but one of the high-affinity antibodies is the most frequently observed somatic mutation and by itself leads to a 10-fold higher affinity; all other somatic exchanges are irrelevant for affinity selection. In the single case of a high-affinity antibody without this common exchange, high affinity is mediated by a combination of mutations (including a one-codon deletion) in VH and the particular D-JH rearrangement carried by this antibody. The data indicate that the pattern of somatic diversification through hypermutation is shaped by affinity selection, but that only a single point mutation is available in the VH and the VL gene of lambda 1 chain-bearing anti-NP antibodies which by itself leads to an increase of hapten-binding affinity. Based on the analysis of two secondary response antibodies from which somatic mutations in VH and VL have been eliminated, it is also concluded that the recruitment of B cell clones into the pathway of hypermutation involves a mechanism which is not based upon affinity differences towards the antigen.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.