Evidence map›Paper›PMID 31380850›Full record

ArticleJournal of visualized experiments : JoVE2019

Ex Vivo Oculomotor Slice Culture from Embryonic GFP-Expressing Mice for Time-Lapse Imaging of Oculomotor Nerve Outgrowth.

Mary C Whitman, Jessica L Bell, Elaine H Nguyen, Elizabeth C Engle

Open access · greenAbstract readVideo-Audio Media
In one paragraph

Article in Journal of visualized experiments : JoVE, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.2field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Review
  3. TUBB3 and KIF21A in neurodevelopment and disease.Frontiers in neuroscience · 2023
    Review
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Mary C WhitmanDepartment of Ophthalmology, Boston Children's Hospital; Department of Ophthalmology, Harvard Medical School; F.M. Kirby Neurobiology Center, Boston Children's Hospital; Mary.Whitman@childrens.harvard.edu.
Jessica L BellDepartment of Ophthalmology, Boston Children's Hospital; F.M. Kirby Neurobiology Center, Boston Children's Hospital.
Elaine H NguyenDepartment of Ophthalmology, Boston Children's Hospital; F.M. Kirby Neurobiology Center, Boston Children's Hospital.
Elizabeth C EngleDepartment of Ophthalmology, Boston Children's Hospital; Department of Ophthalmology, Harvard Medical School; F.M. Kirby Neurobiology Center, Boston Children's Hospital; Department of Neurology, Boston Children's Hospital; Department of Neurology, Harvard Medical School; Howard Hughes Medical Institute.
Boston Children's Hospital · USHarvard University · US

Funding

Viral ModuleP30EY012196 · NEI · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI MARGARET S LIVINGSTONE · 1998 to 2026
$21.3M
Mass Eye and Ear-Vision Clinical Scientist Development ProgramK12EY016335 · NEI · MASSACHUSETTS EYE AND EAR INFIRMARY · PI Reza Dana · 2004 to 2026
$12.3M
Mouse Neurodevelopmental Behavior CoreU54HD090255 · NICHD · BOSTON CHILDREN'S HOSPITAL · PI POMEROY, SCOTT LOREN · 2016 to 2020
$5.2M
Oculomotor axon guidance in normal and abnormal developmentK08EY027850 · NEI · BOSTON CHILDREN'S HOSPITAL · PI WHITMAN, MARY CATHERINE · 2017 to 2019
$648k
Howard Hughes Medical InstituteNEI NIH HHS K08 EY027850NEI NIH HHS K12 EY016335NEI NIH HHS P30 EY012196NICHD NIH HHS U54 HD090255
6 · The paper itself

Abstract

Accurate eye movements are crucial for vision, but the development of the ocular motor system, especially the molecular pathways controlling axon guidance, has not been fully elucidated. This is partly due to technical limitations of traditional axon guidance assays. To identify additional axon guidance cues influencing the oculomotor nerve, an ex vivo slice assay to image the oculomotor nerve in real-time as it grows towards the eye was developed. E10.5 Isl

Indexed as

AnimalsAxonsAxotomyCulture MediaEye MovementsFemaleGreen Fluorescent ProteinsMiceMice, TransgenicOculomotor MusclesOculomotor NerveOrbitOrgan Culture TechniquesPregnancyReceptors, CXCR4Time-Lapse ImagingCulture MediaGreen Fluorescent ProteinsReceptors, CXCR4

Identifiers

PMID31380850
PMCPMC6771922
OpenAlexW2961632542

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.