Evidence map›Paper›PMID 31380359›Full record

ArticleFrontiers in bioengineering and biotechnology2019

3D Microfluidic Bone Tumor Microenvironment Comprised of Hydroxyapatite/Fibrin Composite.

Jungho Ahn, Jungeun Lim, Norhana Jusoh, Jungseub Lee, Tae-Eun Park, YongTae Kim, Jangho Kim, Noo Li Jeon

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in bioengineering and biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.

0numbers the graph read from it
0cells of the map it votes in
37citing papers in PubMed
3.8field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

37 citing papers in PubMed, 77 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 3 countries.

Jungho AhnDepartment of Mechanical and Aerospace Engineering, Seoul National University, Seoul, South Korea.
Jungeun LimDepartment of Mechanical and Aerospace Engineering, Seoul National University, Seoul, South Korea.
Norhana JusohDepartment of Mechanical and Aerospace Engineering, Seoul National University, Seoul, South Korea.
Jungseub LeeDepartment of Mechanical and Aerospace Engineering, Seoul National University, Seoul, South Korea.
Tae-Eun ParkUlsan National Institute of Science and Technology, Ulsan, South Korea.
YongTae KimGeorge W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, United States.
Jangho KimDepartment of Rural and Biosystems Engineering, Chonnam National University, Gwangju, South Korea.
Noo Li JeonDepartment of Mechanical and Aerospace Engineering, Seoul National University, Seoul, South Korea.
Seoul National University · KRChonnam National University · KRThe Wallace H. Coulter Department of Biomedical Engineering · USUlsan National Institute of Science and Technology · KRUniversity of Technology Malaysia · MY

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bone is one of the most common sites of cancer metastasis, as its fertile microenvironment attracts tumor cells. The unique mechanical properties of bone extracellular matrix (ECM), mainly composed of hydroxyapatite (HA) affect a number of cellular responses in the tumor microenvironment (TME) such as proliferation, migration, viability, and morphology, as well as angiogenic activity, which is related to bone metastasis. In this study, we engineered a bone-mimetic microenvironment to investigate the interactions between the TME and HA using a microfluidic platform designed for culturing tumor cells in 3D bone-mimetic composite of HA and fibrin. We developed a bone metastasis TME model from colorectal cancer (SW620) and gastric cancer (MKN74) cells, which has very poor prognosis but rarely been investigated. The microfluidic platform enabled straightforward formation of 3D TME composed the hydrogel and multiple cell types. This facilitated monitoring of the effect of HA concentration and culture time on the TME. In 3D bone mimicking culture, we found that HA rich microenvironment affects cell viability, proliferation and cancer cell cytoplasmic volume in a manner dependent on the different metastatic cancer cell types and culture duration indicating the spatial heterogeneity (different origin of metastatic cancer) and temporal heterogeneity (growth time of cancer) of TME. We also found that both SW620 and MKN72 cells exhibited significantly reduced migration at higher HA concentration in our platform indicating inhibitory effect of HA in both cancer cells migration. Next, we quantitatively analyzed angiogenic sprouts induced by paracrine factors that secreted by TME and showed paracrine signals from tumor and stromal cell with a high HA concentration resulted in the formation of fewer sprouts. Finally we reconstituted vascularized TME allowing direct interaction between angiogenic sprouts and tumor-stroma microspheroids in a bone-mimicking microenvironment composing a tunable HA/fibrin composite. Our multifarious approach could be applied to drug screening and mechanistic studies of the metastasis, growth, and progression of bone tumors.

Indexed as

angiogenesiscancer metastasisfibrin matrixhydroxyapatitemicrofluidic platformtumor microenvironmentvascularized tumor

Identifiers

PMID31380359
PMCPMC6653063
OpenAlexW2959225239

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.