ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2019
Structurally Tunable Reduced Graphene Oxide Substrate Maintains Mouse Embryonic Stem Cell Pluripotency.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 35 citations in OpenAlex.
- Graphene quantum dots remodel osteogenesis of human periodontal ligament stem cells under inflammatory microenvironment: anFrontiers in bioengineering and biotechnology · 2026Article
- Development of the inner ear and regeneration of hair cells after hearing impairment.Fundamental research · 2025Review
- lncRNAs regulate cell stemness in physiology and pathology during differentiation and development.American journal of stem cells · 2024Review
- Recent progresses in neural tissue engineering using topographic scaffolds.American journal of stem cells · 2024Review
- Generation of human otic neuronal organoids using pluripotent stem cells.Cell proliferation · 2023Article
- Commercial dishes with gelatin-free microstructured inserts for elongated stem cell self-renewal and pluripotency.iScience · 2023Article
- D-Galactose-Induced Accelerated Aging Model on Auditory Cortical Neurons by Regulating Oxidative Stress and Apoptosis in Vitro.The journal of nutrition, health & aging · 2022Article
- The influence of reduced graphene oxide on stem cells: a perspective in peripheral nerve regeneration.Regenerative biomaterials · 2021Review
- Bioinspired Soft Robots Based on the Moisture-Responsive Graphene Oxide.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- MgFe-LDH Nanoparticles: A Promising Leukemia Inhibitory Factor Replacement for Self-Renewal and Pluripotency Maintenance in Cultured Mouse Embryonic Stem Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Sirtuin-3 Protects Cochlear Hair Cells Against Noise-Induced DamageFrontiers in cell and developmental biology · 2021Article
- The GDF11 Promotes Nerve Regeneration After Sciatic Nerve Injury in Adult Rats by Promoting Axon Growth and Inhibiting Neuronal Apoptosis.Frontiers in bioengineering and biotechnology · 2021Article
- Familial Temperature-Sensitive Auditory Neuropathy: Distinctive Clinical Courses Caused by Variants of theFrontiers in cell and developmental biology · 2021Article
- Ultrasound-microbubble cavitation facilitates adeno-associated virus mediated cochlear gene transfection across the round-window membrane.Bioengineering & translational medicine · 2021Article
- Layer-Number-Dependent Effects of Graphene Oxide on the Pluripotency of Mouse Embryonic Stem Cells Through the Regulation of the Interaction Between the Extracellular Matrix and Integrins.International journal of nanomedicine · 2021Article
- Atrial Natriuretic Peptide Promotes Neurite Outgrowth and Survival of Cochlear Spiral Ganglion NeuronsFrontiers in cell and developmental biology · 2021Article
- Disruption of Glutamate Release and Uptake-Related Protein Expression After Noise-Induced Synaptopathy in the Cochlea.Frontiers in cell and developmental biology · 2021Article
- A Model of Waardenburg Syndrome Using Patient-Derived iPSCs With aFrontiers in cell and developmental biology · 2021Article
- Cochlear Sox2Frontiers in cell and developmental biology · 2021Article
- Promising Applications of Nanoparticles in the Treatment of Hearing Loss.Frontiers in cell and developmental biology · 2021Review
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Culturing embryonic stem cells (ESCs) in vitro usually requires animal-derived trophoblast cells, which may cause pathogenic and immune reactions; moreover, the poor repeatability between batches hinders the clinical application of ESCs. Therefore, it is essential to synthesize a xenogeneic-free and chemically well-defined biomaterial substrate for maintaining ESC pluripotency. Herein, the effects of structurally tunable reduced graphene oxide (RGO) substrates with different physicochemical properties on ESC pluripotency are studied. Colony formation and CCK-8 assays show that the RGO substrate with an average 30 µm pore size promotes cell survival and proliferation. The unannealed RGO substrate promotes ESC proliferation significantly better than the annealed substrate due to the interfacial hydrophilic groups. The RGO substrate can also maintain ESC for a long time. Additionally, immunofluorescence staining shows that ESCs cultured on an RGO substrate highly express E-cadherin and β-catenin, whereas after being modified by Dickkopf-related protein 1, the RGO substrate is unable to sustain ESC pluripotency. Furthermore, the cell line that interferes with E-cadherin is also unable to maintain pluripotency. These results confirm that the RGO substrate maintains ESC pluripotency by promoting E-cadherin-mediated cell-cell interaction and Wnt signaling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.