ArticleMolecular ecology resources2019
Establishing arthropod community composition using metabarcoding: Surprising inconsistencies between soil samples and preservative ethanol and homogenate from Malaise trap catches.
Article in Molecular ecology resources, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 104 citations in OpenAlex.
- Simultaneous identification of multiple thrips species (Thysanoptera: Thripidae) from sticky traps using DNA metabarcoding.Journal of economic entomology · 2026Article
- Properties and Limitations of eDNA Substrates for Terrestrial Animal Monitoring.Molecular ecology resources · 2026Review
- 16S rRNA sequence dataset for the identification of the French hoverflies (Diptera, Syrphidae).Biodiversity data journal · 2026Article
- Article
- Maximizing Identification Precision of Hymenoptera and Brachycera (Diptera) With a Non-Destructive DNA Metabarcoding Approach.Ecology and evolution · 2025Article
- Article
- Identification of potential insect ecological interactions using a metabarcoding approach.PeerJ · 2025Article
- Environmental gradients and optimal fixation time revealed with DNA metabarcoding of benthic sample fixative.Scientific reports · 2024Article
- A pile of pipelines: An overview of the bioinformatics software for metabarcoding data analyses.Molecular ecology resources · 2024Review
- Three steps towards comparability and standardization among molecular methods for characterizing insect communities.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Article
- Towards holistic insect monitoring: species discovery, description, identification and traits for all insects.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Diversity of Parasitoid Wasps and Comparison of Sampling Strategies in Rice Fields Using Metabarcoding.Insects · 2024Article
- Arthropod diversity in the alpine tundra using metabarcoding: Spatial and temporal differences in alpha- and beta-diversity.Ecology and evolution · 2024Article
- Decontamination protocols affect the internal microbiota of ticks.Parasites & vectors · 2023Article
- Optimizing insect metabarcoding using replicated mock communities.Methods in ecology and evolution · 2023Article
- Monitoring insect biodiversity and comparison of sampling strategies using metabarcoding: A case study in the Yanshan Mountains, China.Ecology and evolution · 2023Article
- Article
- Repeated subsamples during DNA extraction reveal increased diversity estimates in DNA metabarcoding of Malaise traps.Ecology and evolution · 2022Article
- Toward global integration of biodiversity big data: a harmonized metabarcode data generation module for terrestrial arthropods.GigaScience · 2022Article
- Comparison of traditional and DNA metabarcoding samples for monitoring tropical soil arthropods (Formicidae, Collembola and Isoptera).Scientific reports · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
DNA metabarcoding allows the analysis of insect communities faster and more efficiently than ever before. However, metabarcoding can be conducted through several approaches, and the consistency of results across methods has rarely been studied. We compare the results obtained by DNA metabarcoding of the same communities using two different markers - COI and 16S - and three different sampling methods: (a) homogenized Malaise trap samples (homogenate), (b) preservative ethanol from the same samples, and (c) soil samples. Our results indicate that COI and 16S offer partly complementary information on Malaise trap samples, with each marker detecting a significant number of species not detected by the other. Different sampling methods offer highly divergent estimates of community composition. The community recovered from preservative ethanol of Malaise trap samples is significantly different from that recovered from homogenate. Small and weakly sclerotized insects tend to be overrepresented in ethanol while strong and large taxa are overrepresented in homogenate. For soil samples, highly degenerate COI primers pick up large amounts of nontarget DNA and only 16S provides adequate analyses of insect diversity. However, even with 16S, very little overlap in molecular operational taxonomic unit (MOTU) content was found between the trap and the soil samples. Our results demonstrate that none of the tested sampling approaches is satisfactory on its own. For instance, DNA extraction from preservative ethanol is not a valid replacement for destructive bulk extraction but a complement. In future metabarcoding studies, both should ideally be used together to achieve comprehensive representation of the target community.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.