Evidence map›Paper›PMID 31378699›Full record

ArticleAdvances in biological regulation2019

A synthetic biological approach to reconstitution of inositide signaling pathways in bacteria.

Bradley P Clarke, Brandon L Logeman, Andrew T Hale, Zigmund Luka, John D York

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advances in biological regulation, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.6field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Inositol phosphate kinases in the eukaryote landscape.Advances in biological regulation · 2021
    Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Bradley P ClarkeDepartment of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN, USA.
Brandon L LogemanDepartment of Pharmacology and Cancer Biology, Duke University, Durham, NC, USA.
Andrew T HaleDepartment of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN, USA.
Zigmund LukaDepartment of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN, USA.
John D YorkDepartment of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN, USA; Department of Pharmacology and Cancer Biology, Duke University, Durham, NC, USA. Electronic address: john.york@vanderbilt.edu.
Vanderbilt University · USDuke University · US

Funding

The Role of Inositol Signaling in Human DiseaseR01HL055672 · NHLBI · WASHINGTON UNIVERSITY · PI YORK, JOHN D. · 1996 to 2012
$5.4M
Inositol hexakisphosphate regulation of N-terminal acetyltransferasesR01GM124404 · NIGMS · VANDERBILT UNIVERSITY · PI YORK, JOHN D. · 2017 to 2020
$1.6M
Alterations in the sulfur assimilation pathway mediates iron metabolismF30HL143826 · NHLBI · VANDERBILT UNIVERSITY · PI HALE, ANDREW T · 2018 to 2020
$99k
Howard Hughes Medical InstituteNHLBI NIH HHS F30 HL143826NHLBI NIH HHS R01 HL055672NIGMS NIH HHS R01 GM124404
6 · The paper itself

Abstract

Inositide lipid (PIP) and soluble (IP) signaling pathways produce essential cellular codes conserved in eukaryotes. In many cases, deconvoluting metabolic and functional aspects of individual pathways are confounded by promiscuity and multiplicity of PIP and IP kinases and phosphatases. We report a molecular genetic approach that reconstitutes eukaryotic inositide lipid and soluble pathways in a prokaryotic cell which inherently lack inositide kinases and phosphatases in their genome. By expressing synthetic cassettes of eukaryotic genes, we have reconstructed the heterologous formation of a range of inositide lipids, including PI(3)P, PI(4,5)P

Indexed as

Escherichia coliPhosphatidylinositolsSynthetic BiologySignal TransductionPhosphatidylinositolsCell signalingInositol phosphatePhosphatidylinositolSynthetic biology

Identifiers

PMID31378699
PMCPMC7216163
OpenAlexW2966264001

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.