Evidence map›Paper›PMID 31378674›Full record

ArticleStem cell reports2019

Targeted Derivation of Organotypic Glucose- and GLP-1-Responsive β Cells Prior to Transplantation into Diabetic Recipients.

Yaxi Zhu, Jason M Tonne, Qian Liu, Claire A Schreiber, Zhiguang Zhou, Kuntol Rakshit, Aleksey V Matveyenko, Andre Terzic, Dennis Wigle, Yogish C Kudva and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.7field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 2 countries.

Yaxi ZhuDepartment of Molecular Medicine, Mayo Clinic, College of Medicine, 200 First Street SW, Rochester, MN 55905, USA; Institute of Metabolism and Endocrinology, The Second Xiangya Hospital, Key Laboratory of Diabetes Immunology, Ministry of Education, Central South University, National Clinical Research Center for Metabolic Diseases, Changsha, Hunan, China.
Jason M TonneDepartment of Molecular Medicine, Mayo Clinic, College of Medicine, 200 First Street SW, Rochester, MN 55905, USA.
Qian LiuDepartment of Orthopedics, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
Claire A SchreiberDepartment of Molecular Medicine, Mayo Clinic, College of Medicine, 200 First Street SW, Rochester, MN 55905, USA.
Zhiguang ZhouInstitute of Metabolism and Endocrinology, The Second Xiangya Hospital, Key Laboratory of Diabetes Immunology, Ministry of Education, Central South University, National Clinical Research Center for Metabolic Diseases, Changsha, Hunan, China.
Kuntol RakshitDepartment of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, USA.
Aleksey V MatveyenkoDepartment of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, USA; Center for Regenerative Medicine, Mayo Clinic, Rochester, MN, USA.
Andre TerzicCenter for Regenerative Medicine, Mayo Clinic, Rochester, MN, USA.
Dennis WigleCenter for Regenerative Medicine, Mayo Clinic, Rochester, MN, USA; Division of Thoracic Surgery, Mayo Clinic, Rochester, MN, USA.
Yogish C KudvaDivision of Endocrinology, Mayo Clinic, Rochester, MN, USA.
Yasuhiro IkedaDepartment of Molecular Medicine, Mayo Clinic, College of Medicine, 200 First Street SW, Rochester, MN 55905, USA; Center for Regenerative Medicine, Mayo Clinic, Rochester, MN, USA. Electronic address: yasuhiro.ikeda@astrazeneca.com.
Mayo Clinic in Arizona · USCentral South University · CNMayo Clinic · USMayo Clinic in Florida · USSecond Xiangya Hospital of Central South University · CN

Funding

Longitudinal In Vivo Imaging of NIS Expressing Stem CellsR43GM112316 · NIGMS · IMANIS LIFE SCIENCES, LLC · PI IKEDA, YASUHIRO, SUKSANPAISAN, LUKKANA · 2015 to 2015
$203k
NIGMS NIH HHS R43 GM112316
6 · The paper itself

Abstract

Generation of functional β cells from pluripotent sources would accelerate diagnostic and therapeutic applications for diabetes research and therapy. However, it has been challenging to generate competent β cells with dynamic insulin-secretory capacity to glucose and incretin stimulations. We introduced transcription factors, critical for β-cell development and function, in differentiating human induced pluripotent stem cells (PSCs) and assessed the impact on the functionality of derived β-cell (psBC) progeny. A perifusion system revealed stepwise transduction of the PDX1, NEUROG3, and MAFA triad (PNM) enabled in vitro generation of psBCs with glucose and GLP-1 responsiveness within 3 weeks. PNM transduction upregulated genes associated with glucose sensing, insulin secretion, and β-cell maturation. In recipient diabetic mice, PNM-transduced psBCs showed glucose-responsive insulin secretion as early as 1 week post transplantation. Thus, enhanced pre-emptive β-cell specification of PSCs by PNM drives generation of glucose- and incretin-responsive psBCs in vitro, offering a competent tissue-primed biotherapy.

Indexed as

AnimalsBasic Helix-Loop-Helix ProteinsCell DifferentiationC-PeptideDiabetes Mellitus, ExperimentalGene Expression RegulationGlucagon-Like Peptide 1GlucoseGlucose Tolerance TestHomeobox Protein Nkx-2.2Homeodomain ProteinsHumansInduced Pluripotent Stem CellsInsulin-Secreting CellsInsulin SecretionMaf Transcription Factors, LargeBasic Helix-Loop-Helix ProteinsC-PeptideGlucagon-Like Peptide 1GlucoseHomeobox Protein Nkx-2.2Homeodomain ProteinsMAFA protein, humanMaf Transcription Factors, LargeNerve Tissue ProteinsNEUROG3 protein, humanNkx6-1 protein, mousepancreatic and duodenal homeobox 1 proteinTrans-ActivatorsZebrafish ProteinsiPSCMAFANEUROG3PDX1reprogrammingtranscription factorβ-cell regeneration

Identifiers

PMID31378674
PMCPMC6700523
OpenAlexW2965141994

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.