Evidence map›Paper›PMID 31377975›Full record

ArticleWorld journal of pediatrics : WJP2019

Attenuated MuV-S79 as vector stably expressing foreign gene.

Duo Zhou, Meng-Ying Zhu, Yi-Long Wang, Xiao-Qiang Hao, Dong-Ming Zhou, Rong-Xian Liu, Chu-Di Zhang, Chu-Fan Qu, Zheng-Yan Zhao

Abstract read
PubMed Publisher
In one paragraph

Article in World journal of pediatrics : WJP, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Duo ZhouZhejiang University School of Medicine, Hangzhou, Zhejiang, China.
Meng-Ying ZhuZhejiang University School of Medicine, Hangzhou, Zhejiang, China.
Yi-Long WangDepartment of Neurology, Children's Hospital, Zhejiang University School of Medicine, Hangzhou, 310052, China.
Xiao-Qiang HaoZhejiang University School of Medicine, Hangzhou, Zhejiang, China.
Dong-Ming ZhouChildren's Hospital, Zhejiang University School of Medicine, Hangzhou, 310052, China.
Rong-Xian LiuZhejiang University School of Medicine, Hangzhou, Zhejiang, China.
Chu-Di ZhangZhejiang University School of Medicine, Hangzhou, Zhejiang, China.
Chu-Fan QuZhejiang University School of Medicine, Hangzhou, Zhejiang, China.
Zheng-Yan ZhaoZhejiang University School of Medicine, Hangzhou, Zhejiang, China. zhaozy@zju.edu.cn.
Zhejiang University · CNChildren's Hospital of Zhejiang University · CN

Funding

LQ19H100005 LQ19H100005
6 · The paper itself

Abstract

backgroundTo describe mumps virus (MuV) used as a vector to express enhanced green fluorescent protein (EGFP) or red fluorescent protein (RFP) genes.

methodsMolecular cloning technique was applied to establish the cDNA clones of recombinant mumps viruses (rMuVs). rMuVs were recovered based on our reverse genetic system of MuV-S79. The properties of rMuVs were determined by growth curve, plaque assay, fluorescent microscopy and determination of fluorescent intensity.

resultsThree recombinant viruses replicated well in Vero cells and similarly as parental rMuV-S79, expressed heterologous genes in high levels, and were genetically stable in at least 15 passages.

conclusionrMuV-S79 is a promising platform to accommodate foreign genes like marker genes, other antigens and immunomodulators for addressing various diseases.

Indexed as

Reverse GeneticsAnimalsChlorocebus aethiopsCloning, MolecularGreen Fluorescent ProteinsLuminescent ProteinsMumps virusRed Fluorescent ProteinVero Cellsenhanced green fluorescent proteinGreen Fluorescent ProteinsLuminescent ProteinsRed Fluorescent ProteinForeign genesMumps virusReverse genetic system

Identifiers

PMID31377975
OpenAlexW2966147678

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.