Evidence map›Paper›PMID 31376280›Full record

ArticleJournal of experimental botany2019

Low-fluence blue light-induced phosphorylation of Zmphot1 mediates the first positive phototropism.

Hiromi Suzuki, Tomokazu Koshiba, Chiharu Fujita, Yoshio Yamauchi, Taro Kimura, Toshiaki Isobe, Tatsuya Sakai, Masato Taoka, Takashi Okamoto

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of experimental botany, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.2field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 12 citations in OpenAlex.

  1. The modulation of light quality on carotenoids in maize (Food chemistry. Molecular sciences · 2022
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Hiromi SuzukiDepartment of Biological Sciences, Tokyo Metropolitan University, Hachioji-shi, Tokyo, Japan.
Tomokazu KoshibaDepartment of Biological Sciences, Tokyo Metropolitan University, Hachioji-shi, Tokyo, Japan.
Chiharu FujitaDepartment of Chemistry, Tokyo Metropolitan University, Hachioji-shi, Tokyo, Japan.
Yoshio YamauchiDepartment of Chemistry, Tokyo Metropolitan University, Hachioji-shi, Tokyo, Japan.
Taro KimuraJapan Society for the Promotion of Science, Kojimachi Business Center Building, Chiyoda-ku, Tokyo, Japan.
Toshiaki IsobeDepartment of Chemistry, Tokyo Metropolitan University, Hachioji-shi, Tokyo, Japan.
Tatsuya SakaiGraduate School of Science and Technology, Niigata University, Niigata-shi, Niigata, Japan.
Masato TaokaDepartment of Chemistry, Tokyo Metropolitan University, Hachioji-shi, Tokyo, Japan.
Takashi OkamotoDepartment of Biological Sciences, Tokyo Metropolitan University, Hachioji-shi, Tokyo, Japan.
Tokyo Metropolitan University · JPJapan Society for the Promotion of Science · JPNiigata University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Phototropin1 (phot1) perceives low- to high-fluence blue light stimuli and mediates both the first and second positive phototropisms. High-fluence blue light is known to induce autophosphorylation of phot1, leading to the second positive phototropism. However, the phosphorylation status of phot1 by low-fluence blue light that induces the first positive phototropism had not been observed. Here, we conducted a phosphoproteomic analysis of maize coleoptiles to investigate the fluence-dependent phosphorylation status of Zmphot1. High-fluence blue light induced phosphorylation of Zmphot1 at several sites. Notably, low-fluence blue light significantly increased the phosphorylation level of Ser291 in Zmphot1. Furthermore, Ser291-phosphorylated and Ser369Ser376-diphosphorylated peptides were found to be more abundant in the low-fluence blue light-irradiated sides than in the shaded sides of coleoptiles. The roles of these phosphorylation events in phototropism were explored by heterologous expression of ZmPHOT1 in the Arabidopsis thaliana phot1phot2 mutant. The first positive phototropism was restored in wild-type ZmPHOT1-expressing plants; however, plants expressing S291A-ZmPHOT1 or S369AS376A-ZmPHOT1 showed significantly reduced complementation rates. All transgenic plants tested in this study exhibited a normal second positive phototropism. These findings provide the first indication that low-fluence blue light induces phosphorylation of Zmphot1 and that this induced phosphorylation is crucial for the first positive phototropism.

Indexed as

LightArabidopsisArabidopsis ProteinsPhosphorylationPhototropismPlants, Genetically ModifiedZea maysArabidopsis ProteinsArabidopsis thalianacoleoptilefirst positive phototropismlow-fluence blue lightphosphoproteomicsphototropin1Zea maysZmphot1

Identifiers

PMID31376280
PMCPMC6812725
OpenAlexW2966283628

What OpenQuestion holds

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LicenceCC BY-NC
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.