ArticleJournal of industrial microbiology & biotechnology2019
Ultra-high-throughput picoliter-droplet microfluidics screening of the industrial cellulase-producing filamentous fungus Trichoderma reesei.
Article in Journal of industrial microbiology & biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- High-throughput droplet screening of filamentous fungi on solid substrates.RSC chemical biology · 2026Article
- Semi-automated strain engineering and screening in the thermophilic fungusApplied and environmental microbiology · 2026Article
- Genetically encoded biosensors enabled high-throughput screening of microbial cell factories.Engineering microbiology · 2026Review
- Size-independent and automated single-colony-resolution microdroplet dispensing.Lab on a chip · 2025Article
- Rapid filamentous fungi gene knockout identification through high-throughput droplet microfluidics.Analytica chimica acta · 2025Article
- Considerations for Domestication of Novel Strains of Filamentous Fungi.ACS synthetic biology · 2025Review
- Regulation of genes encoding polysaccharide-degrading enzymes in Penicillium.Applied microbiology and biotechnology · 2024Review
- Evolution and screening of Trichoderma reesei mutants for secreted protein production at elevated temperature.Journal of industrial microbiology & biotechnology · 2024Article
- Current achievements, strategies, obstacles, and overcoming the challenges of the protein engineering in Pichia pastoris expression system.World journal of microbiology & biotechnology · 2023Review
- Application of Atmospheric and Room-Temperature Plasma (ARTP) to Microbial Breeding.Current issues in molecular biology · 2023Review
- A microfluidic device for simultaneous detection of enzyme secretion and elongation of a single hypha.Frontiers in microbiology · 2023Article
- High-throughput screening of microbial strains in large-scale microfluidic droplets.Frontiers in bioengineering and biotechnology · 2023Article
- Fungi-on-a-Chip: microfluidic platforms for single-cell studies on fungi.FEMS microbiology reviews · 2022Review
- Growth Stimulation, Phosphate Resolution, and Resistance to Fungal Pathogens of Some Endogenous Fungal Strains in the Rhizospheres of Medicinal Plants in Vietnam.Molecules (Basel, Switzerland) · 2022Article
- Ultrahigh-throughput screening of industrial enzyme-producing strains by droplet-based microfluidic system.Journal of industrial microbiology & biotechnology · 2022Article
- Microfluidic screening and genomic mutation identification for enhancing cellulase production in Pichia pastoris.Biotechnology for biofuels and bioproducts · 2022Article
- In Situ Corn Fiber Conversion for Ethanol Improvement by the Addition of a Novel Lignocellulolytic Enzyme Cocktail.Journal of fungi (Basel, Switzerland) · 2022Article
- 7-Aminocoumarin-4-acetic Acid as a Fluorescent Probe for Detecting Bacterial Dipeptidyl Peptidase Activities in Water-in-Oil Droplets and in Bulk.Analytical chemistry · 2022Article
- Droplet Microfluidics for Microbial Biotechnology.Advances in biochemical engineering/biotechnology · 2022Article
- Development of a flow cytometry-based plating-free system for strain engineering in industrial fungi.Applied microbiology and biotechnology · 2022Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The selection of improved producers among the huge number of variants in mutant libraries is a key issue in filamentous fungi of industrial biotechnology. Here, we developed a droplet-based microfluidic high-throughput screening platform for selection of high-cellulase producers from filamentous fungus Trichoderma reesei. The screening system used a fluorogenic assay to measure amount of cellulase and its activity. The key effectors such as cellulase-inducing medium, spore germination, droplet cultivation time, droplet fluorescence signal detection, and droplet cell sorting were studied. An artificial pre-mixed library of high- and low-cellulase-producing T. reesei strains was screened successfully to verify the feasibility of our method. Finally, two cellulase hyperproducers exhibiting improvements in cellulase activity of 27% and 46% were isolated from an atmospheric and room-temperature plasma (ARTP)-mutated library. This high-throughput screening system could be applied to the engineering of T. reesei strains and other industrially valuable protein-producing filamentous fungi.
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