ArticleNature communications2019
Cryoprotectant enables structural control of porous scaffolds for exploration of cellular mechano-responsiveness in 3D.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers, 1 of them a synthesis that pooled it.
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Who cites it
76 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Application of Additive Manufacturing in Assisted Reproductive Techniques: What Is the Evidence? A Clinical and Technical Systematic Review of the Literature.Medicina (Kaunas, Lithuania) · 2024Pooled it
- Interpretable AI-driven materiomics to decode microenvironmental cues for stem cell immunomodulation.Bioactive materials · 2027Article
- ICE-CAP: ice crystal engineering for cryogel architecture programming. Modulation of material properties with ice-binding polymers.Materials horizons · 2026Article
- Immune-epigenetic convergence in biomaterial-guided tissue regeneration.Materials today. Bio · 2026Article
- Rational design of immunological programs for functional regenerative niches.Materials today. Bio · 2026Review
- Biomimetic injectable engineered hierarchical porous microspheres for enhanced synergistic cell therapy of critical limb ischemia.Bioactive materials · 2026Article
- Dynamic stiffness enables stage-specific properties mediating functional endothelialization on vascular implants.Science advances · 2026Article
- Regulatory mechanism of stem cell scaffold porosity and stiffness on the processes of intramembranous ossification and bone regeneration.Stem cell research & therapy · 2026Article
- PLA/hydroxyapatite composite scaffolds fabricated by digital light processing for bone regeneration.Journal of materials science. Materials in medicine · 2026Article
- Age-mimicking hydrogel stiffness recapitulates the mechanical niche of the hippocampus to regulate neural stem cell senescence.Materials today. Bio · 2026Article
- Viscoelastic hydrogel primed CAR-macrophage for pulmonary fibrosis treatment.Nature communications · 2026Article
- Potential of organ-on-a-chip in advancing synthetic extracellular matrix technology for bone tissue engineering in dentistry (Review).Biomedical reports · 2026Review
- Engineered macroporous gelatin scaffolds enhance lymph node fibroblastic reticular cell identity and enable diabetogenic T cell immunomodulation.Biomaterials · 2026Article
- Physical cues influence the construction of the osteoimmune microenvironment: focus on the hematoma period.Regenerative biomaterials · 2026Review
- Sustainable synthesis of amino-cellulose nanofibers for biomaterial platforms.Science advances · 2025Article
- High-mobility network hydrogel microsphere system to combat chondrocyte senescence for enhanced cartilage repair and regeneration.Materials today. Bio · 2025Article
- Healing of tendon-related diseases: insights from macrophage regulation.Military Medical Research · 2025Review
- Hydrogel-Mediated Preservation of Live Tumor Explants for Drug Development in Peritoneal Metastases.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Extracellular matrix dynamics in tumor immunoregulation: from tumor microenvironment to immunotherapy.Journal of hematology & oncology · 2025Review
- The mechanism and prospect of exogenous promoters enhancement Anammox at low temperatures: a review.World journal of microbiology & biotechnology · 2025Review
16 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite the wide applications, systematic mechanobiological investigation of 3D porous scaffolds has yet to be performed due to the lack of methodologies for decoupling the complex interplay between structural and mechanical properties. Here, we discover the regulatory effect of cryoprotectants on ice crystal growth and use this property to realize separate control of the scaffold pore size and stiffness. Fibroblasts and macrophages are sensitive to both structural and mechanical properties of the gelatin scaffolds, particularly to pore sizes. Interestingly, macrophages within smaller and softer pores exhibit pro-inflammatory phenotype, whereas anti-inflammatory phenotype is induced by larger and stiffer pores. The structure-regulated cellular mechano-responsiveness is attributed to the physical confinement caused by pores or osmotic pressure. Finally, in vivo stimulation of endogenous fibroblasts and macrophages by implanted scaffolds produce mechano-responses similar to the corresponding cells in vitro, indicating that the physical properties of scaffolds can be leveraged to modulate tissue regeneration.
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