Evidence map›Paper›PMID 31375667›Full record

ArticleNature communications2019

A murine neonatal model of necrotizing enterocolitis caused by anemia and red blood cell transfusions.

Krishnan MohanKumar, Kopperuncholan Namachivayam, Tanjing Song, Byeong Jake Cha, Andrea Slate, Jeanne E Hendrickson, Hua Pan, Samuel A Wickline, Joo-Yeun Oh, Rakesh P Patel and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
83citing papers in PubMed, 2 pooled it
20.8field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

83 citing papers in PubMed, 2 syntheses or guidelines pooled it, 113 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Trial
  4. Trial
  5. Trial
  6. Article
  7. Article
  8. Article
  9. Anemia and Transfusion in Preclinical Models of Neonatology.Methods in molecular biology (Clifton, N.J.) · 2026
    Article
  10. Review
  11. Article
  12. Article
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  14. Article
  15. Review
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  18. Tight junction proteins and intestinal health in preterm infants.Journal of neonatal-perinatal medicine · 2025
    Review
  19. Article
  20. [Regional mesenteric oxygen saturation during blood transfusion in premature neonates].Revista medica del Instituto Mexicano del Seguro Social · 2025
    Observational

23 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 4 institutions in 1 country.

Krishnan MohanKumarDepartment of Pediatrics, Morsani College of Medicine, University of South Florida, Tampa, FL, 33612, USA.
Kopperuncholan NamachivayamDepartment of Pediatrics, Morsani College of Medicine, University of South Florida, Tampa, FL, 33612, USA.
Tanjing SongDepartment of Pediatrics, Morsani College of Medicine, University of South Florida, Tampa, FL, 33612, USA.
Byeong Jake ChaDepartment of Molecular Pharmacology and Physiology, Morsani College of Medicine, University of South Florida, Tampa, FL, 33612, USA.
Andrea SlateDepartment of Comparative Medicine, University of South Florida, Tampa, FL, 33612, USA.
Jeanne E HendricksonDepartment of Laboratory Medicine, Yale School of Medicine, New Haven, CT, 06520, USA.ORCID http://orcid.org/0000-0002-7928-3132
Hua PanDepartment of Cardiology, Morsani College of Medicine, University of South Florida, Tampa, FL, 33629, USA.
Samuel A WicklineDepartment of Cardiology, Morsani College of Medicine, University of South Florida, Tampa, FL, 33629, USA.
Joo-Yeun OhCenter for Free Radical Biology, University of Alabama at Birmingham, Birmingham, AL, 35294, USA.
Rakesh P PatelCenter for Free Radical Biology, University of Alabama at Birmingham, Birmingham, AL, 35294, USA.
Ling HeDepartment of Pediatrics, Johns Hopkins University, Baltimore, MD, 21287, USA.
Benjamin A TorresDepartment of Pediatrics, Morsani College of Medicine, University of South Florida, Tampa, FL, 33612, USA.
Akhil MaheshwariDepartment of Pediatrics, Morsani College of Medicine, University of South Florida, Tampa, FL, 33612, USA. akhil@jhmi.edu.ORCID http://orcid.org/0000-0003-1502-7663
University of South Florida · USJohns Hopkins University · USUniversity of Alabama at Birmingham · USYale University · US

Funding

Yale Clinical and Translational Science Award (U Component)UL1TR001863 · NCATS · YALE UNIVERSITY · PI John H. Krystal, LUCILA OHNO-MACHADO · 2016 to 2026
$102.9M
METHODS IN MOLECULAR IMAGING AND TARGETED THERAPEUTICSR01HL073646 · NHLBI · WASHINGTON UNIVERSITY · PI WICKLINE, SAMUEL A · 2004 to 2016
$13.7M
Coactivator P300 promotes hepatic steatosis in obesityR01DK120309 · NIDDK · JOHNS HOPKINS UNIVERSITY · PI HE, LING, YU, LIQING · 2019 to 2023
$2.7M
Prevention and Treatment of Acute Kidney InjuryR01DK102691 · NIDDK · WASHINGTON UNIVERSITY · PI WICKLINE, SAMUEL A · 2015 to 2018
$2.0M
Activation of the cAMP-PKA pathway antagonizes metformin actionR01DK107641 · NIDDK · JOHNS HOPKINS UNIVERSITY · PI HE, LING · 2016 to 2020
$1.8M
Inhibition of NF-kB with siRNA/Peptide Nanoparticles for Arthritis TreatmentR01AR067491 · NIAMS · WASHINGTON UNIVERSITY · PI PHAM, CHRISTINE T., WICKLINE, SAMUEL A · 2015 to 2019
$1.8M
Effect of Platelet Transfusions on Neonatal Intestinal InjuryR01HL133022 · NHLBI · UNIVERSITY OF SOUTH FLORIDA · PI KRISHNAN, MOHAN KUMAR · 2017 to 2020
$1.6M
Red Cell Transfusion-associated Necrotizing Enterocolitis in Premature InfantsR01HL124078 · NHLBI · UNIVERSITY OF SOUTH FLORIDA · PI KRISHNAN, MOHAN KUMAR · 2015 to 2018
$1.5M
NCATS NIH HHS UL1 TR001863NHLBI NIH HHS R01 HL073646NHLBI NIH HHS R01 HL124078NHLBI NIH HHS R01 HL133022NIAMS NIH HHS R01 AR067491NIDDK NIH HHS R01 DK102691NIDDK NIH HHS R01 DK107641NIDDK NIH HHS R01 DK120309
6 · The paper itself

Abstract

Necrotizing enterocolitis (NEC) is an idiopathic, inflammatory bowel necrosis of premature infants. Clinical studies have linked NEC with antecedent red blood cell (RBC) transfusions, but the underlying mechanisms are unclear. Here we report a neonatal murine model to investigate this association. C57BL/6 mouse pups rendered anemic by timed phlebotomy and then given RBC transfusions develop NEC-like intestinal injury with prominent necrosis, inflammation, and submucosal edema/separation of the lamina propria in the ileocecal region and colon within 12-24 h. The anemic intestine is infiltrated by inflammatory macrophages, which are activated in situ by RBC transfusions via a Toll-like receptor (TLR)-4-mediated mechanism and cause bowel injury. Chelation of RBC degradation products with haptoglobin, absence of TLR4, macrophage depletion, and inhibition of macrophage activation is protective. Intestinal injury worsens with increasing severity and the duration of anemia prior to transfusion, indicating a need for the re-evaluation of current transfusion guidelines for premature infants.

Indexed as

AnemiaAnimalsAnimals, NewbornCecumColonDisease Models, AnimalEnterocolitis, NecrotizingErythrocyte TransfusionHumansIleumInfant, NewbornInfant, Newborn, DiseasesInfant, PrematureIntestinal MucosaMacrophagesMiceTlr4 protein, mouseToll-Like Receptor 4

Identifiers

PMID31375667
PMCPMC6677753
OpenAlexW2965785686

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.