ArticleJournal of virology2019
Establishment of a Virulent Full-Length cDNA Clone for Type I Feline Coronavirus Strain C3663.
Article in Journal of virology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
- Bridging the antiviral gap: A BAC-based non-infectious SARS-CoV-2 replicon reveals potent antisense gapmers.Molecular therapy. Nucleic acids · 2026Article
- Mutations in feline infectious peritonitis virus nonstructural protein 14/16 methyltransferase attenuate the pathogenicity of the virus in cats.Journal of virology · 2025Article
- Assembly and Mutagenesis of Human Coronavirus OC43 Genomes in Yeast via Transformation-Associated Recombination.Bio-protocol · 2025Article
- A colloidal gold immunochromatographic test strip based on mAbs anti-N protein to detect feline coronavirus.Microbiology spectrum · 2025Article
- Article
- Application of versatile reverse genetics system for feline coronavirus.Microbiology spectrum · 2025Article
- A yeast-based reverse genetics system to generate HCoV-OC43 reporter viruses encoding an eighth subgenomic RNA.Journal of virology · 2025Article
- In Vitro Evaluation of Aryl Hydrocarbon Receptor Involvement in Feline Coronavirus Infection.Viruses · 2025Article
- Development and characterization of reverse genetics systems of feline infectious peritonitis virus for antiviral research.Veterinary research · 2024Article
- Isolation and Genomic Characteristics of a Novel Pathogenicity Type I Feline Coronavirus in Mainland China.Transboundary and emerging diseases · 2024Article
- An optimized circular polymerase extension reaction-based method for functional analysis of SARS-CoV-2.Virology journal · 2023Article
- An Optimized Circular Polymerase Extension Reaction-based Method for Functional Analysis of SARS-CoV-2.bioRxiv : the preprint server for biology · 2022Article
- Reverse Genetics with a Full-Length Infectious cDNA Clone of Bovine Torovirus.Journal of virology · 2022Article
- Establishment of Full-Length cDNA Clones and an Efficient Oral Infection Model for Feline Coronavirus in Cats.Journal of virology · 2021Article
- Construction, Characterization and Application of Recombinant Porcine Deltacoronavirus Expressing Nanoluciferase.Viruses · 2021Article
- Systematizing the genomic order and relatedness in the open reading frames (ORFs) of the coronaviruses.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2021Article
- Establishment of a reverse genetics system for SARS-CoV-2 using circular polymerase extension reaction.Cell reports · 2021Article
- Recent Progress in Torovirus Molecular Biology.Viruses · 2021Review
- Identification and phylogenetic analysis of two canine coronavirus strains.Animal diseases · 2021Article
- High-throughput viral microneutralization method for feline coronavirus using image cytometry.Journal of virological methods · 2020Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Feline infectious peritonitis (FIP) is one of the most important infectious diseases in cats and is caused by feline coronavirus (FCoV). Tissue culture-adapted type I FCoV shows reduced FIP induction in experimental infections, which complicates the understanding of FIP pathogenesis caused by type I FCoV. We previously found that the type I FCoV strain C3663 efficiently induces FIP in specific-pathogen-free cats through the naturally infectious route. In this study, we employed a bacterial artificial chromosome-based reverse genetics system to gain more insights into FIP caused by the C3633 strain. We successfully generated recombinant virus (rC3663) from Fcwf-4 cells transfected with infectious cDNA that showed growth kinetics similar to those shown by the parental virus. Next, we constructed a reporter C3663 virus carrying the nanoluciferase (Nluc) gene to measure viral replication with high sensitivity. The inhibitory effects of different compounds against rC3663-Nluc could be measured within 24 h postinfection. Furthermore, we found that A72 cells derived from canine fibroblasts permitted FCoV replication without apparent cytopathic effects. Thus, our reporter virus is useful for uncovering the infectivity of type I FCoV in different cell lines, including canine-derived cells. Surprisingly, we uncovered aberrant viral RNA transcription of rC3663 in A72 cells. Overall, we succeeded in obtaining infectious cDNA clones derived from type I FCoV that retained its virulence. Our recombinant FCoVs are powerful tools for increasing our understanding of the viral life cycle and pathogenesis of FIP-inducing type I FCoV.
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