ArticleThe European journal of neuroscience2019
Transcriptional profiling aligned with in situ expression image analysis reveals mosaically expressed molecular markers for GABA neuron sub-groups in the ventral tegmental area.
Article in The European journal of neuroscience, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Therapeutic potential and underlying mechanisms of engineered young plasma-derived exosomes in Alzheimer's disease.Bioactive materials · 2027Article
- Stress resilience is associated with transcriptional remodeling in the VTA.Cell reports · 2026Article
- Ventral tegmental area GABA neurons integrate positive and negative valence.Nature communications · 2025Article
- Stress resilience is associated with transcriptional remodeling in the VTA.bioRxiv : the preprint server for biology · 2025Article
- Ventral tegmental area GABA neurons integrate positive and negative valence.bioRxiv : the preprint server for biology · 2025Article
- Flexible updating of reward and punishment contingencies by VTA GABA neurons.Current biology : CB · 2025Article
- Promoter demethylation of cerebellin 2 by ten-eleven translocation 3 contributes to peripheral sensitization in trigeminal neuropathic pain of mice.The journal of headache and pain · 2025Article
- Ventral tegmental area interneurons revisited: GABA and glutamate projection neurons make local synapses.eLife · 2025Article
- Ventral tegmental area interneurons revisited: GABA and glutamate projection neurons make local synapses.bioRxiv : the preprint server for biology · 2025Article
- Ethanol blocks a novel form of iLTD, but not iLTP of inhibitory inputs to VTA GABA neurons.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2023Article
- GPR101 loss promotes insulin resistance and diet-induced obesity risk.Neuroscience applied · 2023Article
- A specific circuit in the midbrain detects stress and induces restorative sleep.Science (New York, N.Y.) · 2022Article
- Early Life Social Stress Causes Sex- and Region-Dependent Dopaminergic Changes that Are Prevented by Minocycline.Molecular neurobiology · 2022Article
- An atlas of transcriptionally defined cell populations in the rat ventral tegmental area.Cell reports · 2022Article
- An emerging map of glutamate delta 1 receptors in the forebrain.Neuropharmacology · 2021Review
- Heterogeneous somatostatin-expressing neuron population in mouse ventral tegmental area.eLife · 2020Article
- Differential Level of RXFP3 Expression in Dopaminergic Neurons Within the Arcuate Nucleus, Dorsomedial Hypothalamus and Ventral Tegmental Area of RXFP3-Cre/tdTomato Mice.Frontiers in neuroscience · 2020Article
- Transcriptional profiling aligned with in situ expression image analysis reveals mosaically expressed molecular markers for GABA neuron sub-groups in the ventral tegmental area.The European journal of neuroscience · 2019Article
- VTA GABA Neurons at the Interface of Stress and Reward.Frontiers in neural circuits · 2019Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
γ-Aminobutyric acid (GABA) neurons in the ventral tegmental area (VTA) provide local inhibitory control of dopamine neuron activity and send long-range projections to several target regions including the nucleus accumbens. They play diverse roles in reward and aversion, suggesting that they be comprised of several functionally distinct sub-groups, but our understanding of this diversity has been limited by a lack of molecular markers that might provide genetic entry points for cell type-specific investigations. To address this, we conducted transcriptional profiling of GABA neurons and dopamine neurons using immunoprecipitation of tagged polyribosomes (RiboTag) and RNAseq. First, we directly compared these two transcriptomes in order to obtain a list of genes enriched in GABA neurons compared with dopamine neurons. Next, we created a novel bioinformatic approach, that used the PANTHER (Protein ANalysis THrough Evolutionary Relationships) gene ontology database and VTA gene expression data from the Allen Mouse Brain Atlas, from which we obtained 6 candidate genes: Cbln4, Rxfp3, Rora, Gpr101, Trh and Nrp2. As a final step, we verified the selective expression of these candidate genes in sub-groups of GABA neurons in the VTA (and neighbouring substantia nigra pars compacta) using immunolabelling. Taken together, our study provides a valuable toolbox for the future investigation of GABA neuron sub-groups in the VTA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.