ArticleAmerican journal of physiology. Heart and circulatory physiology2019
Optimized protocols for isolation, fixation, and flow cytometric characterization of leukocytes in ischemic hearts.
Article in American journal of physiology. Heart and circulatory physiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Who cites it
19 citing papers in PubMed, 23 citations in OpenAlex.
- Cytomegalovirus Latency Fuels Inflammation Following Myocardial Infarction.Circulation research · 2026Article
- Extracellular Vesicles Mediate Activation and Trafficking of Splenic Immune Cells to the Heart Post-Myocardial Infarction.bioRxiv : the preprint server for biology · 2026Article
- Activation of β2-Adrenergic Receptor Alleviates Viral Myocarditis by Regulating Energy Metabolism in Monocyte-derived Macrophages via the AMPK Pathway.Inflammation · 2026Article
- Isolation of Immune Cells from Skeletal Muscles for Flow Cytometry.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Self-assembled extracellular matrix-lipid nanoparticle composite for site-specific siRNA delivery to improve cardiac repair post-myocardial infarction.Materials today. Bio · 2025Article
- CD206Circulation · 2025Article
- AIMP3 maintains cardiac homeostasis by regulating the editing activity of methionyl-tRNA synthetase.Nature cardiovascular research · 2025Article
- Cross-species single-cell RNA-seq analysis reveals disparate and conserved cardiac and extracardiac inflammatory responses upon heart injury.Communications biology · 2024Article
- Formoterol Reduces the Pro-Inflammatory Phenotype by Enhancing the Activity of Glutaminase in Monocyte-Derived Macrophages in the CVB3-Induced Viral Myocarditis.Immunity, inflammation and disease · 2024Article
- YTHDF1 is pivotal for maintenance of cardiac homeostasis.Journal of molecular and cellular cardiology · 2024Article
- Guidelines on antibody use in physiology research.American journal of physiology. Renal physiology · 2024Article
- Artificial intelligence in imaging flow cytometry.Frontiers in bioinformatics · 2023Article
- TNFR1 Contributes to Activation-Induced Cell Death of Pathological CD4JACC. Basic to translational science · 2022Article
- Estrogen Receptor-β Agonists Modulate T-Lymphocyte Activation and Ameliorate Left Ventricular Remodeling During Chronic Heart Failure.Circulation. Heart failure · 2022Article
- BEX1 is a critical determinant of viral myocarditis.PLoS pathogens · 2022Article
- CD4Frontiers in cardiovascular medicine · 2022Article
- Early Inflammation in Muscular Dystrophy Differs between Limb and Respiratory Muscles and Increases with Dystrophic Severity.The American journal of pathology · 2021Article
- Fibroblast growth factor-inducible 14 mediates macrophage infiltration in heart to promote pressure overload-induced cardiac dysfunction.Life sciences · 2020Article
- Adaptive immunity-driven inflammation and cardiovascular disease.American journal of physiology. Heart and circulatory physiology · 2019Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
Immune activation post-myocardial infarction is an orchestrated sequence of cellular responses to effect tissue repair and healing. However, excessive and dysregulated inflammation can result in left ventricular remodeling and pathological alterations in the structural and mechanical attributes of the heart. Identification of key pathways and critical cellular mediators of inflammation is thus essential to design immunomodulatory therapies for myocardial infarction and ischemic heart failure. Despite this, the experimental approaches to isolate mononuclear cells from the heart are diverse, and detailed protocols to enable maximum yield of live cells in the shortest time possible are not readily available. Here, we describe optimized protocols for the isolation, fixation, and flow cytometric characterization of cardiac CD45
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.