Evidence map›Paper›PMID 31373375›Full record

ArticleThe Journal of nutrition2019

High Dietary Iron Disrupts Iron Homeostasis and Induces Amyloid-β and Phospho-τ Expression in the Hippocampus of Adult Wild-Type and APP/PS1 Transgenic Mice.

Min Chen, Jiashuo Zheng, Guohao Liu, Chong Zeng, En Xu, Wenjie Zhu, Gregory J Anderson, Huijun Chen

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of nutrition, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
1.6field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 25 citations in OpenAlex.

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  9. Tip60/Kat5 may be a novel candidate histone acetyltransferase for the regulation of liver iron localization via acetylation.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2022
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Min ChenJiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University, Nanjing, China.
Jiashuo ZhengJiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University, Nanjing, China.
Guohao LiuJiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University, Nanjing, China.
Chong ZengJiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University, Nanjing, China.
En XuJiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University, Nanjing, China.
Wenjie ZhuJiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University, Nanjing, China.
Gregory J AndersonQIMR Berghofer Medical Research Institute, Brisbane, QLD, Australia.
Huijun ChenJiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University, Nanjing, China.
Nanjing University · CNQIMR Berghofer Medical Research Institute · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundBrain iron deposition is a feature of Alzheimer disease and may contribute to its development. However, the relative contribution of dietary iron remains unclear.

objectivesWe investigated the impact of high dietary iron on brain pathological changes and cognitive function in adult wild-type (WT) mice and amyloid precursor protein/presenilin 1 (APP/PS1) double transgenic mice.

methodsMale WT mice and APP/PS1 mice aged 10 wk were fed either a control diet (66 mg Fe/kg) (WT-Ctrl and APP/PS1-Ctrl) or a high iron diet (14 g Fe/kg) (WT-High Fe and APP/PS1-High Fe) for 20 wk. Iron concentrations in brain regions were measured by atomic absorption spectrophotometry. Brain iron staining and amyloid-β (Aβ) immunostaining were performed. Protein expressions in the hippocampus were determined by immunoblotting. Superoxide dismutase (SOD) activity and malondialdehyde concentration were examined. Cognitive functions were tested with the Morris water maze system.

resultsIn the hippocampus, APP/PS1-High Fe mice had significantly higher iron concentration (2.5-fold) and ferritin (2.0-fold) than APP/PS1-Ctrl mice (P < 0.001), and WT-High Fe mice had significantly higher ferritin (2.0-fold) than WT-Ctrl mice (P < 0.001). Interestingly, APP/PS1 mice had significantly higher iron concentration (2-3-fold) and ferritin (2-2.5-fold) than WT mice fed either diet (P < 0.001). Histological analysis indicated that iron accumulated in the hippocampal dentate gyrus region in APP/PS1 mice, consistent with the pattern of Aβ deposition. For both mouse strains, iron treatment induced Aβ and phospho-τ expression (1.5-3-fold) in the hippocampus, but had little impact on oxidative stress and cognitive function. Furthermore, APP/PS1 mice had significantly lower SOD activity and higher malondialdehyde concentration than WT mice in the hippocampus (P < 0.0001), paralleled by apparent cognitive dysfunction.

conclusionsDietary iron overload induces iron disorder and Aβ and phospho-τ expression in the hippocampus of adult WT and APP/PS1 transgenic mice.

Indexed as

HomeostasisAlzheimer DiseaseAmyloid beta-PeptidesAnimalsDietGrowthHippocampusIronMaleMiceMice, TransgenicOxidative StressPhosphorylationPresenilin-1tau ProteinsAmyloid beta-PeptidesIronMapt protein, mousePresenilin-1presenilin 1, mousetau ProteinsAlzheimer diseaseamyloid-βAPP/PS1 micecognitive functiondietary iron overloadhippocampusoxidative stress

Identifiers

PMID31373375
PMCPMC6887700
OpenAlexW2966695557

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.