ArticleThe Analyst2019
Influence of antibody immobilization strategy on carbon electrode immunoarrays.
Article in The Analyst, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 57 citations in OpenAlex.
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- Electrochemical Immunosensor for the Quantification of Galectin-3 in Saliva.Sensors and actuators. B, Chemical · 2024Article
- Hybrid Impedimetric Biosensors for Express Protein Markers Detection.Micromachines · 2024Review
- A review of antibody, aptamer, and nanomaterials synergistic systems for an amplified electrochemical signal.Frontiers in bioengineering and biotechnology · 2024Review
- Novel Sensitive Electrochemical Immunosensor Development for the Selective Detection of HopQBiosensors · 2023Article
- Development of a Cyclic Voltammetry-Based Method for the Detection of Antigens and Antibodies as a Novel Strategy for Syphilis Diagnosis.International journal of environmental research and public health · 2022Article
- Performance of electrochemical immunoassays for clinical diagnostics of SARS-CoV-2 based on selective nucleocapsid N protein detection: Boron-doped diamond, gold and glassy carbon evaluation.Biosensors & bioelectronics · 2022Article
- The Effect of Preconditioning Strategies on the Adsorption of Model Proteins onto Screen-Printed Carbon Electrodes.Sensors (Basel, Switzerland) · 2022Article
- Gold-based nanoplatform for a rapid lateral flow immunochromatographic test assay for gluten detection.BMC biomedical engineering · 2022Article
- Advances in Nanomaterial-based Biosensors for Determination of Glycated Hemoglobin.Current topics in medicinal chemistry · 2022Article
- Synthesis and grafting of diazonium tosylates for thermoplastic electrode immunosensors.Analytical methods : advancing methods and applications · 2021Article
- Determination of p53 biomarker using an electrochemical immunoassay based on layer-by-layer films with NiFeMikrochimica acta · 2020Article
- Review
- Sub-zeptomole Detection of Biomarker Proteins Using a Microfluidic Immunoarray with Nanostructured Sensors.Analytical chemistry · 2020Article
- Screen Printed Based Impedimetric Immunosensor for Rapid Detection ofSensors (Basel, Switzerland) · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 5 countries.
Funding
Abstract
We report here the influence of antibody immobilization strategy for protein immunosensors on screen printed carbon electrode arrays in terms of antibody binding activity, analytical sensitivity, limit of detection, and stability. Horseradish peroxidase (HRP) was the model analyte with anti-HRP immobilized on the sensors, and HRP activity was used for detection. Covalently immobilized anti-HRP antibodies on electrodes coated with chitosan, electrochemically reduced graphene oxide (rGO), and dense gold nanoparticle (AuNP) films had only 20-30% of the total immobilized antibodies active for binding. Active antibodies increased to 60% with passively adsorbed antibodies on bare electrodes, to 85% with oriented antibodies using protein A covalently immobilized on AuNP-coated carbon electrode, and to 98% when attached to protein A passively adsorbed onto bare electrodes. Passively adsorbed antibodies on bare electrodes lost activity in 1-2 days, but antibodies immobilized using other strategies remained relatively stable after 5 days. Covalent immobilization gave limits of detection (LOD) of 40 fg mL
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.