ArticlePakistan journal of medical sciences
Isolation and culture of dental pulp stem cells from permanent and deciduous teeth.
Article in Pakistan journal of medical sciences. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 36 citations in OpenAlex.
- Regenerative capacity of human dental pulp stem cells versus their exosomes on surgically induced submandibular gland defects in rats.BMC oral health · 2026Article
- Synergistic effects of collagen membrane and mineral trioxide aggregate on odontogenic differentiation and mineralization of human dental pulp stem cells: an in vitro study.BMC oral health · 2026Article
- From Extraction to Cryobanking: Which Critical Process Parameters Genuinely Improve DPSC Production?Pharmaceuticals (Basel, Switzerland) · 2026Article
- No simple way to averaging out: Pooled mesenchymal stromal cells do not reflect average donor characteristics.Regenerative therapy · 2025Article
- Validated methods for isolation and qualification of mesenchymal stromal/stem cells from different sources.Journal of translational medicine · 2025Review
- The Secretome of Human Deciduous Tooth-Derived Mesenchymal Stem Cells Enhances In Vitro Wound Healing and Modulates Inflammation.Pharmaceutics · 2025Article
- Effect of the pulp harvesting method on the viability of human dental pulp stem cells and their odontogenic differentiation potential.Cell and tissue banking · 2025Article
- Temporal Profiling of Cellular and Molecular Processes in Osteodifferentiation of Dental Pulp Stem Cells.Biology · 2025Article
- Stem cell therapy for regenerating periodontal bony defects: A narrative review.Journal of advanced periodontology & implant dentistry · 2025Review
- The potential of exosomes from adipose-derived stromal-vascular fraction in Increasing Migration Activity of Human Dental Pulp Stromal Cells (in vitroThe Saudi dental journal · 2024Article
- Comparative evaluation of the osteogenic capacity of second-generation platelet concentrates on dental pulp stem cells - AnJournal of conservative dentistry and endodontics · 2024Article
- Article
- Isolation and Culture of Primary Human Dental Pulp Cells-A Description of Technical and Methodological Steps to Maximise Predictability and Yield.Methods and protocols · 2024Article
- Mesenchymal Stromal Cells Derived from Dental Tissues: Immunomodulatory Properties and Clinical Potential.International journal of molecular sciences · 2024Review
- Stem cell sources from human biological waste material: a role for the umbilical cord and dental pulp stem cells for regenerative medicine.Human cell · 2023Review
- Analysis of Thrombin-Activated Platelet-Derived Exosome (T-aPDE) Potential for Dental Pulp Regeneration: In-Vitro Study.European journal of dentistry · 2023Article
- Towards Induction of Angiogenesis in Dental Pulp Stem Cells Using Chitosan-Based Hydrogels Releasing Basic Fibroblast Growth Factor.BioMed research international · 2022Article
- Article
- Ultrasonic techniques to obtain dental pulp from impacted third molars.Journal of clinical and experimental dentistry · 2021Article
- Therapeutic Functions of Stem Cells from Oral Cavity: An Update.International journal of molecular sciences · 2020Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo isolate dental pulp mesenchymal stem cells (MSCs) from non-infected human permanent and deciduous teeth.
methodsIt was an in-vitro experimental study. Human teeth were collected from 13 apparently healthy subjects including nine adults and four children. After decoronation dental pulps were extirpated from teeth and cultured via explant method in a stem cell defined media. Data was analyzed by descriptive statistics.
resultsAs above MSCs emerged exhibiting fibroblast-like morphology. In vitro culture was positive for 100% (9/9) and 75% (3/4) of the permanent and deciduous teeth respectively. First cell appeared from deciduous teeth pulp in 10±6.2 days while permanent teeth pulp took 12.4±3.7 days. Together, 26.6±3.6 and 24.5±3.5 days were required for permanent and deciduous tooth pulp stem cells to be ready for further assays.
conclusionsThe protocol we developed is easy and consistent and can be used to generate reliable source of MScs for engineering of calcified and non-calcified tissue for regenerative medicine approaches.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.