ArticleScientific reports2019
Microbial diversity analysis and screening for novel xylanase enzymes from the sediment of the Lobios Hot Spring in Spain.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 55 citations in OpenAlex.
- Fungal-bacterial interaction networks in lignocellulose degradation: an integrated ecological and mechanistic perspective.Biotechnology for biofuels and bioproducts · 2026Review
- Exploring the biotechnological potential of terrestrial hot spring microbiomes for COEnvironmental microbiome · 2026Article
- Molecular cloning and characterization of a GH10 thermophilic xylanase from hot spring and its potential application in promoting probiotic growth.BMC biotechnology · 2026Article
- Metagenomics and its impact on environmental and therapeutic microbiology.Archives of microbiology · 2025Review
- NGS-Based Metagenomics Depicting Taxonomic and Functional Insights into North-Western Himalayan Hot Springs.Indian journal of microbiology · 2024Article
- Heterologous expression and structure prediction of a xylanase identified from a compost metagenomic library.Applied microbiology and biotechnology · 2024Article
- Precision enzyme discovery through targeted mining of metagenomic data.Natural products and bioprospecting · 2024Review
- Review
- Mining thermophiles for biotechnologically relevant enzymes: evaluating the potential of European and Caucasian hot springs.Extremophiles : life under extreme conditions · 2023Review
- Characterization of a GH10 extremely thermophilic xylanase from the metagenome of hot spring for prebiotic production.Scientific reports · 2023Article
- Metabolic versatility ofFrontiers in microbiology · 2023Article
- Complex organic matter degradation by secondary consumers in chemolithoautotrophy-based subsurface geothermal ecosystems.PloS one · 2023Article
- Prokaryotes of renowned Karlovy Vary (Carlsbad) thermal springs: phylogenetic and cultivation analysis.Environmental microbiome · 2022Article
- An essential role for tungsten in the ecology and evolution of a previously uncultivated lineage of anaerobic, thermophilic Archaea.Nature communications · 2022Article
- Bioprospecting for Thermozymes and Characterization of a Novel Lipolytic Thermozyme Belonging to the SGNH/GDSL Family of Hydrolases.International journal of molecular sciences · 2022Article
- Mining Marine Metagenomes Revealed a Quorum-Quenching Lactonase with Improved Biochemical Properties That Inhibits the Food Spoilage Bacterium Pseudomonas fluorescens.Applied and environmental microbiology · 2022Article
- Thermophiles and carbohydrate-active enzymes (CAZymes) in biofilm microbial consortia that decompose lignocellulosic plant litters at high temperatures.Scientific reports · 2022Article
- Breeding of a thermostable xylanase-producing strain ofFrontiers in bioengineering and biotechnology · 2022Article
- Phenotype Switching in Metal-Tolerant Bacteria Isolated from a Hyperaccumulator Plant.Biology · 2021Article
- Hemicellulosic biomass conversion by Moroccan hot spring3 Biotech · 2021Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, we describe the metagenome composition of a microbial community in a hot spring sediment as well as a sequence-based and function-based screening of the metagenome for identification of novel xylanases. The sediment was collected from the Lobios Hot Spring located in the province of Ourense (Spain). Environmental DNA was extracted and sequenced using Illumina technology, and a total of 3.6 Gbp of clean paired reads was produced. A taxonomic classification that was obtained by comparison to the NCBI protein nr database revealed a dominance of Bacteria (93%), followed by Archaea (6%). The most abundant bacterial phylum was Acidobacteria (25%), while Thaumarchaeota (5%) was the main archaeal phylum. Reads were assembled into contigs. Open reading frames (ORFs) predicted on these contigs were searched by BLAST against the CAZy database to retrieve xylanase encoding ORFs. A metagenomic fosmid library of approximately 150,000 clones was constructed to identify functional genes encoding thermostable xylanase enzymes. Function-based screening revealed a novel xylanase-encoding gene (XynA3), which was successfully expressed in E. coli BL21. The resulting protein (41 kDa), a member of glycoside hydrolase family 11 was purified and biochemically characterized. The highest activity was measured at 80 °C and pH 6.5. The protein was extremely thermostable and showed 94% remaining activity after incubation at 60 °C for 24 h and over 70% remaining activity after incubation at 70 °C for 24 h. Xylanolytic activity of the XynA3 enzyme was stimulated in the presence of β-mercaptoethanol, dithiothreitol and Fe
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