ArticleProceedings of the National Academy of Sciences of the United States of America2019
Building a synthetic mechanosensitive signaling pathway in compartmentalized artificial cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
55 citing papers in PubMed, 128 citations in OpenAlex.
- Membrane-Associated Biomolecules for Synthetic Cell Signalling.Chembiochem : a European journal of chemical biology · 2026Review
- The Shape of Things to Come: α-Helical Membrane Protein Folding on the Ribosome.Chemical reviews · 2026Review
- Bottom-Up Coacervate-Based Artificial Cells: Integrating Cellular Hallmarks into Complex Life-Like Systems.Angewandte Chemie (International ed. in English) · 2026Review
- Engineering synthetic cells with intramembrane domains possessing distinct bilayer asymmetries.Nature communications · 2026Article
- Affordable, cleanroom-free millifluidic production of targeted lipid nanocarriersLab on a chip · 2026Article
- pH-responsive synthetic cells for controlled protein synthesis and release.bioRxiv : the preprint server for biology · 2025Article
- Cation-Controlled Assembly, Activity, and Organization of Biomimetic DNA Receptors in Synthetic Cell Membranes.Journal of the American Chemical Society · 2025Article
- Microfluidic Production of Spatially Structured Biomimetic Microgels as Compartmentalized Artificial Cells.Small science · 2025Article
- Droplet-supported liquid-liquid lateral phase separation as a step to floating protein heterostructures.Nature communications · 2025Article
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- Adaptive metal ion transport and metalloregulation-driven differentiation in pluripotent synthetic cells.Nature chemistry · 2025Article
- Force versus Response: Methods for Activating and Characterizing Mechanosensitive Ion Channels and GPCRs.Advanced healthcare materials · 2024Review
- Manipulation of encapsulated artificial phospholipid membranes using sub-micellar lysolipid concentrations.Communications chemistry · 2024Article
- Biomimetic behaviors in hydrogel artificial cells through embedded organelles.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Noise Minimization in Cell-Free Gene Expression.ACS synthetic biology · 2023Article
- Engineering Tissue-Scale Properties with Synthetic Cells: Forging One from Many.ACS synthetic biology · 2023Review
- DNA-Origami Line-Actants Control Domain Organization and Fission in Synthetic Membranes.Journal of the American Chemical Society · 2023Article
- Engineering transmembrane signal transduction in synthetic membranes using two-component systems.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- A simple method to make, trap and deform a vesicle in a gel.Scientific reports · 2023Article
- Cell-sized asymmetric phospholipid-amphiphilic protein vesicles with growth, fission, and molecule transportation.iScience · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
To date, reconstitution of one of the fundamental methods of cell communication, the signaling pathway, has been unaddressed in the bottom-up construction of artificial cells (ACs). Such developments are needed to increase the functionality and biomimicry of ACs, accelerating their translation and application in biotechnology. Here, we report the construction of a de novo synthetic signaling pathway in microscale nested vesicles. Vesicle-cell models respond to external calcium signals through activation of an intracellular interaction between phospholipase A2 and a mechanosensitive channel present in the internal membranes, triggering content mixing between compartments and controlling cell fluorescence. Emulsion-based approaches to AC construction are therefore shown to be ideal for the quick design and testing of new signaling networks and can readily include synthetic molecules difficult to introduce to biological cells. This work represents a foundation for the engineering of multicompartment-spanning designer pathways that can be utilized to control downstream events inside an AC, leading to the assembly of micromachines capable of sensing and responding to changes in their local environment.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.