ArticleMethods in enzymology2019
Combinatorial control of gene function with wavelength-selective caged morpholinos.
Article in Methods in enzymology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Self-transfecting GMO-PMO chimera targetingMolecular therapy. Nucleic acids · 2023Article
- Bicyclic Caged Morpholino Oligonucleotides for Optical Gene Silencing.Chembiochem : a European journal of chemical biology · 2022Article
- Conditional gene knockdowns in sea urchins using caged morpholinos.Developmental biology · 2021Article
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Authors and funding
4 authors.
Funding
Abstract
Caged morpholino oligonucleotides (cMOs) are useful research tools in developmental biology because they allow spatiotemporal control of gene expression in whole organisms. While cMOs are usually triggered by light of a single wavelength, the introduction of spectrally distinct chromophores can enable combinatorial regulation of multiple genes. This chapter describes the general principles and methods of wavelength-selective cMO design and synthesis from commercially available reagents. Synthetic protocols for the linkers and the two-step cMO assembly are described in detail, as well as the microinjection and photoactivation techniques. Following these protocols, spectrally separated cyclic cMOs for multiple genes of interest can be prepared, enabling their inhibition in zebrafish embryos and other animal models.
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