ArticleBMC biotechnology2019
Lack of specificity associated with using molecular beacons in loop mediated amplification assays.
Article in BMC biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Multiplex PCR Fluorescence Method for Detection of Genetically Modified Maize Strains.Current issues in molecular biology · 2026Article
- Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of High-Burden Viral Diseases in Resource-Limited Countries.Pathogens (Basel, Switzerland) · 2026Review
- Ultrasensitive and Multiplexed Target Detection Strategy Based on Photocleavable Mass Tags and Mass Signal Amplification.Nanomaterials (Basel, Switzerland) · 2025Article
- Fast-Flu: RT-RPA-CRISPR/Cas12a assisted one-step platform for rapid influenza B virus detection.Microbiology spectrum · 2025Article
- Development of a novel Cas13a/Cas12a-mediated 'one-pot' dual detection assay for genetically modified crops.Journal of advanced research · 2025Article
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- A method to measure molecular hybridization.PloS one · 2024Article
- Development of a real-time recombinase-aided amplification assay for rapid and sensitive detection ofFrontiers in cellular and infection microbiology · 2024Article
- Rapid Detection of DNA and RNA Shrimp Viruses Using CRISPR-Based Diagnostics.Applied and environmental microbiology · 2023Article
- Digital CRISPR Systems for the Next Generation of Nucleic Acid Quantification.Trends in analytical chemistry : TRAC · 2023Article
- Simplified detection of Epstein-Barr virus for diagnosis of endemic Burkitt lymphoma.Blood advances · 2022Article
- Figure of Merit for CRISPR-Based Nucleic Acid-Sensing Systems: Improvement Strategies and Performance Comparison.ACS sensors · 2022Article
- Plasmonic LAMP: Improving the Detection Specificity and Sensitivity for SARS-CoV-2 by Plasmonic Sensing of Isothermally Amplified Nucleic Acids.Small (Weinheim an der Bergstrasse, Germany) · 2022Article
- Loop-Mediated Isothermal Amplification: From Theory to Practice.Russian journal of bioorganic chemistry · 2022Article
- Recombinase-Aided Amplification Coupled with Lateral Flow Dipstick for Efficient and Accurate Detection of Porcine Parvovirus.Life (Basel, Switzerland) · 2021Article
- Modified "Allele-Specific qPCR" Method for SNP Genotyping Based on FRET.Frontiers in plant science · 2021Article
- Development of Recombinase Aided Amplification Combined With Disposable Nucleic Acid Test Strip for Rapid Detection of Porcine Circovirus Type 2.Frontiers in veterinary science · 2021Article
- Cleavable hairpin beacon-enhanced fluorescence detection of nucleic acid isothermal amplification and smartphone-based readout.Scientific reports · 2020Article
- Quantitative LAMP and PCR Detection ofInternational journal of food science · 2020Article
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2 authors.
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Abstract
backgroundLoop mediated isothermal amplification of nucleic acid templates is a rapid, sensitive and specific method suitable for molecular diagnostics. However the complexity of primer design and the number of primers involved can lead to false positives from non-specific primer interactions. Standard methods of LAMP detection utilise the increasing concentrations of DNA or inorganic pyrophosphate and therefore lack specificity for identifying the desired LAMP amplification. Molecular beacons used in PCR reactions are target specific and may enhance specificity with LAMP.
resultsWe present a potential molecular beacon approach to LAMP detection targeting the single stranded region between loops, and test this for LAMP molecular beacons targeting the 35S promoter and NOS terminator sequences commonly used in GM crops. From these studies we show that molecular beacons used in LAMP, despite providing a change in fluorescent intensity with amplification, appear not to anneal to specific target sequences and therefore target specificity is not a benefit of this method. However, molecular beacons demonstrate a change in fluorescence which is indicative of LAMP amplification products. We identify the LAMP loop structure as likely to be responsible for this change in signal.
conclusionsMolecular beacons can be used to detect LAMP amplification but do not provide sequence specificity. The method can be used to determine effectively LAMP amplification from other primer-driven events, but does not discriminate between different LAMP amplicons. It is therefore unsuitable for multiplex LAMP reactions due to non-specific detection of LAMP amplification.
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