ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2019
Seeded propagation of α-synuclein aggregation in mouse brain using protein misfolding cyclic amplification.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Amplification of seeding-competent tau aggregates by PMCA in human and experimental tauopathies.Acta neuropathologica communications · 2026Article
- Enteric glia, neuropeptides, and Parkinson's: impacts of calcitonin gene-related peptide on gut alpha synuclein.American journal of physiology. Gastrointestinal and liver physiology · 2026Article
- Oxidative Stress and Lysosomal Dysfunction in Neurodegenerative Diseases: Underlying Mechanisms and Nanotherapeutic Targeting Strategies.Antioxidants (Basel, Switzerland) · 2026Review
- Divalent and Trivalent Metallic Ions Differentially Affect Alpha-Synuclein Aggregation.ACS chemical neuroscience · 2025Article
- Subclinical detection of misfolded alpha-synuclein in the blood of M83 mice.Acta neuropathologica · 2025Article
- Development of an efficient expression system for human chaperone BiP in Pichia pastoris: production optimization and functional validation.Microbial cell factories · 2025Article
- Protease-Sensitive and -Resistant Forms of Human and Murine Alpha-Synucleins in Distinct Brain Regions of Transgenic Mice (M83) Expressing the Human Mutated A53T Protein.Biomolecules · 2023Article
- Gut mucosal cells transfer α-synuclein to the vagus nerve.JCI insight · 2023Article
- Crocin Protects Malathion-Induced Striatal Biochemical Deficits by Inhibiting Apoptosis and Increasing α-Synuclein in Rats' Striatum.Journal of molecular neuroscience : MN · 2022Article
- Chronic Exposure to Paraquat Induces Alpha-Synuclein Pathogenic Modifications inInternational journal of molecular sciences · 2021Article
- Parkinson-like early autonomic dysfunction induced by vagal application of DOPAL in rats.CNS neuroscience & therapeutics · 2021Article
- Development of α-Synuclein Real-Time Quaking-Induced Conversion as a Diagnostic Method for α-Synucleinopathies.Frontiers in aging neuroscience · 2021Review
- Article
- Analysis of Protein Conformational Strains-A Key for New Diagnostic Methods of Human Diseases.International journal of molecular sciences · 2020Article
- Structural heterogeneity of α-synuclein fibrils amplified from patient brain extracts.Nature communications · 2019Article
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7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
α-Synuclein (α-syn) protein aggregation is associated with several neurodegenerative disorders collectively referred to as synucleinopathies, including Parkinson's disease. We used protein misfolding cyclic amplification (PMCA) to study α-syn aggregation in brain homogenates of wild-type or transgenic mice expressing normal (D line) or A53T mutant (M83 line) human α-syn. We found that sonication-incubation cycles of M83 mouse brain gradually produce large quantities of SDS-resistant α-syn aggregates, involving both human and mouse proteins. These PMCA products, containing partially proteinase K-resistant α-syn species, are competent to accelerate the onset of neurologic symptoms after intracerebral inoculation to young M83 mice and to seed aggregate formation of α-syn following PMCA, including in D and wild-type mouse brain substrates. PMCA seeding activity in the M83 diseased brain correlates positively with regions mostly targeted by the α-syn pathology in this model. Our data indicate that similar to prions, PMCA can reproduce some characteristics of α-syn aggregation and seeded propagation
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