ArticleViruses2019
Rapid Detection of Peste des Petits Ruminants Virus (PPRV) Nucleic Acid Using a Novel Low-Cost Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for Future Use in Nascent PPR Eradication Programme.
Article in Viruses, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 30 citations in OpenAlex.
- Epidemiology of Peste des Petits Ruminants in Nigeria: A Review.Frontiers in veterinary science · 2022Pooled it
- Two Pentaplex Real-Time Fluorescent PCR Assays for Simultaneous Detection of Ten Ovine Respiratory and Reproductive Pathogens.Viruses · 2026Article
- Development and Validation of an RT-Droplet Digital PCR Assay for Detection of Peste des Petits Ruminants.Veterinary sciences · 2026Article
- A systematic review of peste des petits ruminants in Bangladesh: Epidemiology, diagnostics, risk factors, vaccination, and gaps toward eradication.Veterinary world · 2026Review
- Development of a double-antibody sandwich ELISA for detecting Peste des petits ruminants virus infection.Applied microbiology and biotechnology · 2026Article
- Detection of Lineage IV Peste Des Petits Ruminants Virus by RT-qPCR Assay via Targeting the Hemagglutinin Gene.Viruses · 2025Article
- Enhanced Recovery and Detection of Highly Infectious Animal Disease Viruses by Virus Capture Using NanotrapViruses · 2024Article
- Comparative study of HA and HNB staining RT-LAMP assays for peste des petits ruminants virus detection in West African Dwarf goats.Tropical animal health and production · 2023Article
- Simultaneous detection and identification of Peste des petits ruminants Virus Lineages II and IV by MCA-Based real-time quantitative RT-PCR assay within single reaction.BMC veterinary research · 2023Article
- Investigation of foot and mouth disease virus and other animal pathogens in cattle, buffaloes and goats at the interface with Akagera National Park 2017 - 2020.BMC veterinary research · 2022Article
- Article
- Pheno- and genotypic characterization and identification of novel subtypes of Peste des Petits Ruminants virus in domestic and captive wild goats in Northern Iraq.BMC microbiology · 2021Article
- Development and Evaluation of a Nested PCR for Improved Diagnosis and Genetic Analysis of Peste des Petits Ruminants Virus (PPRV) for Future Use in Nascent PPR Eradication Programme.Animals : an open access journal from MDPI · 2021Article
- Transboundary Animal Diseases, an Overview of 17 Diseases with Potential for Global Spread and Serious Consequences.Animals : an open access journal from MDPI · 2021Review
- Peste des petits ruminants in large ruminants, camels and unusual hosts.The veterinary quarterly · 2020Review
- "FastCheckViruses · 2020Article
- Review
- Paradigm shift in the diagnosis of peste des petits ruminants: scoping review.Acta veterinaria Scandinavica · 2020Article
- A rapid RT-LAMP assay for the detection of all four lineages of Peste des Petits Ruminants Virus.Journal of virological methods · 2019Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Peste des petits ruminants (PPR) is a disease of small ruminants caused by peste des petits ruminants virus (PPRV), and is endemic in Asia, the Middle East and Africa. Effective control combines the application of early warning systems, accurate laboratory diagnosis and reporting, animal movement restrictions, suitable vaccination and surveillance programs, and the coordination of all these measures by efficient veterinary services. Molecular assays, including conventional reverse transcription-polymerase chain reaction (RT-PCR) and real-time RT-PCR (RT-qPCR) have improved the sensitivity and rapidity of diagnosing PPR. However, currently these assays are only performed within laboratory settings; therefore, the development of field diagnostics for PPR would improve the fast implementation of control policies, particularly when PPR has been targeted to be eradicated by 2030. Loop-mediated isothermal amplification (LAMP) assays are simple to use, rapid, and have sensitivity and specificity within the range of RT-qPCR; and can be performed in the field using disposable consumables and portable equipment. This study describes the development of a novel RT-LAMP assay for the detection of PPRV nucleic acid by targeting the N-protein gene. The RT-LAMP assay was evaluated using cell culture propagated PPRVs, field samples from clinically infected animals and samples from experimentally infected animals encompassing all four lineages (I-IV) of PPRV. The test displayed 100% concordance with RT-qPCR when considering an RT-qPCR cut-off value of C
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