Trial reportPLoS neglected tropical diseases2019
Diagnostic performance of a single and duplicate Kato-Katz, Mini-FLOTAC, FECPAKG2 and qPCR for the detection and quantification of soil-transmitted helminths in three endemic countries.
Trial report in PLoS neglected tropical diseases, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03465488 (Validation of Both Automated Quality Assured Egg Counting System and Molecular Markers for Monitoring the Spread of Anthelmintic Resistance.), which is not on this map. Cited by 69 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Validation of Both Automated Quality Assured Egg Counting System and Molecular Markers for Monitoring the Spread of Anthelmintic Resistance.
Who cites it
69 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Soil-transmitted helminths and schistosome infections in Ethiopia: a systematic review of progress in their control over the past 20 years.Parasites & vectors · 2021Pooled it
- Anthelmintic drugs for treating ascariasis.The Cochrane database of systematic reviews · 2020Pooled it
- A comparison of the diagnostic capability of Kato-Katz and real-time PCR for the assessment of treatment efficacy of ivermectin and albendazole combination against T. trichiura infections.PLoS neglected tropical diseases · 2024Trial
- A randomized assessors-blind clinical trial to evaluate the safety and the efficacy of albendazole alone and in combination with mebendazole or pyrantel for the treatment ofAntimicrobial agents and chemotherapy · 2024Trial
- A general framework to support cost-efficient fecal egg count methods and study design choices for large-scale STH deworming programs-monitoring of therapeutic drug efficacy as a case study.PLoS neglected tropical diseases · 2023Trial
- Individual responses to a single oral dose of albendazole indicate reduced efficacy against soil-transmitted helminths in an area with high drug pressure.PLoS neglected tropical diseases · 2021Trial
- Epidemiology of soil-transmitted helminths following sustained implementation of routine preventive chemotherapy: Demographics and baseline results of a cluster randomised trial in southern Malawi.PLoS neglected tropical diseases · 2021Trial
- Therapeutic efficacy of albendazole against soil-transmitted helminthiasis in children measured by five diagnostic methods.PLoS neglected tropical diseases · 2019Trial
- Molecular identification of hookworm infection in humans, dogs and cats in Lao People's Democratic Republic.Infectious diseases of poverty · 2026Article
- Community survey of the infection, intensity and risk factors associated with schistosomiasis and soil-transmitted helminthiasis in south-eastern Gabon.Scientific reports · 2026Article
- Performance evaluation of the diaxxoPCR system for rapid and user-friendly stool-based diagnosis of trichuriasis, ascariasis and strongyloidiasis in Mozambique.PLoS neglected tropical diseases · 2025Article
- Factors associated with detecting pathogenic intestinal parasites in multiple stool microscopy samples: a retrospective cross-sectional study.Parasitology research · 2025Article
- Harnessing Molecular Techniques to Inform Species-Specific Hookworm Prevalence and Infection Intensity and Detect Strongyloides in Dak Lak Province, Vietnam.The American journal of tropical medicine and hygiene · 2025Article
- Detecting soil-transmitted helminth and Schistosoma mansoni eggs in Kato-Katz stool smear microscopy images: A comprehensive in- and out-of-distribution evaluation of YOLOv7 variants.PLoS neglected tropical diseases · 2025Article
- The Changing Face of Helminth Infections: A 15-Year Temporal Analysis from Eastern Saudi Arabia.Infection and drug resistance · 2025Article
- Assessing the diagnostic value of qPCR forFrontiers in parasitology · 2025Article
- Low prevalence of helminth infection in Ugandan children hospitalized with severe malaria.PloS one · 2025Article
- Community-based prevalence, intensity and risk factors associated with soil-transmitted helminthiases and intestinal schistosomiasis in Apojola, Ogun state, southwest Nigeria.BMC infectious diseases · 2024Article
- Artificial intelligence-based digital pathology for the detection and quantification of soil-transmitted helminths eggs.PLoS neglected tropical diseases · 2024Article
- All that glitters is not gold: An interpretive framework for diagnostic test evaluation using Ascaris lumbricoides as a conceptual example.PLoS neglected tropical diseases · 2024Article
9 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
23 authors.
Funding
Abstract
backgroundBecause the success of deworming programs targeting soil-transmitted helminths (STHs) is evaluated through the periodically assessment of prevalence and infection intensities, the use of the correct diagnostic method is of utmost importance. The STH community has recently published for each phase of a deworming program the minimal criteria that a potential diagnostic method needs to meet, the so-called target product profiles (TPPs). METHODOLOGY: We compared the diagnostic performance of a single Kato-Katz (reference method) with that of other microscopy-based methods (duplicate Kato-Katz, Mini-FLOTAC and FECPAKG2) and one DNA-based method (qPCR) for the detection and quantification of STH infections in three drug efficacy trials in Ethiopia, Lao PDR, and Tanzania. Furthermore, we evaluated a selection of minimal diagnostic criteria of the TPPs. PRINCIPAL
findingsAll diagnostic methods showed a clinical sensitivity of ≥90% for all STH infections of moderate-to-heavy intensities. For infections of very low intensity, only qPCR resulted in a sensitivity that was superior to a single Kato-Katz for all STHs. Compared to the reference method, both Mini-FLOTAC and FECPAKG2 resulted in significantly lower fecal egg counts for some STHs, leading to a substantial underestimation of the infection intensity. For qPCR, there was a positive significant correlation between the egg counts of a single Kato-Katz and the DNA concentration. CONCLUSIONS/SIGNIFICANCE: Our results indicate that the diagnostic performance of a single Kato-Katz is underestimated by the community and that diagnostic specific thresholds to classify intensity of infection are warranted for Mini-FLOTAC, FECPAKG2 and qPCR. When we strictly apply the TPPs, Kato-Katz is the only microscopy-based method that meets the minimal diagnostic criteria for application in the planning, monitoring and evaluation phase of an STH program. qPCR is the only method that could be considered in the phase that aims to seek confirmation for cessation of program.
trial registrationClinicalTrials.gov NCT03465488.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.