Evidence map›Paper›PMID 31368812›Full record

ArticleAmerican journal of respiratory cell and molecular biology2019

Improving the Quality and Reproducibility of Flow Cytometry in the Lung. An Official American Thoracic Society Workshop Report.

Robert M Tighe, Elizabeth F Redente, Yen-Rei Yu, Susanne Herold, Anne I Sperling, Jeffrey L Curtis, Ryan Duggan, Suchitra Swaminathan, Hideki Nakano, William J Zacharias and 6 more

Registry-linked trialAbstract read
In one paragraph

Article in American journal of respiratory cell and molecular biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03034642 (Modulation of Steroid Immunosuppression by Alveolar Efferocytosis), which is not on this map. Cited by 41 papers.

0numbers the graph read from it
0cells of the map it votes in
41citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT03034642 nacompletednot on this map

Modulation of Steroid Immunosuppression by Alveolar Efferocytosis

TypeinterventionalSponsorVA Ann Arbor Healthcare SystemRan2015 to 2021Enrolled60ConditionsPulmonary Disease, Chronic Obstructive, Pneumonia, BacterialArmsBronchoscopy with bilateral bronchoalveolar lavages
3 · Its place in the literature

Who cites it

41 citing papers in PubMed.

  1. Monocyte-derived galectin-1Nature immunology · 2026
    Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Review
  13. Alpha-1 antitrypsin inhibits fractalkine-mediated monocyte-lung endothelial cell interactions.American journal of physiology. Lung cellular and molecular physiology · 2023
    Article
  14. Article
  15. Article
  16. Tissue-resident alveolar macrophages reduce ObioRxiv : the preprint server for biology · 2023
    Article
  17. Multicompartmental analysis of the murine pulmonary immune response by spectral flow cytometry.American journal of physiology. Lung cellular and molecular physiology · 2023
    Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Elizabeth F Redente
Yen-Rei Yu
Suchitra Swaminathan
William J ZachariasORCID 0000-0002-2643-0610
William J JanssenORCID 0000-0002-6397-3454
Christine M Freeman
Ryan R Brinkman
Benjamin D SingerORCID 0000-0001-5775-8427
Claudia V Jakubzick
Alexander V MisharinORCID 0000-0003-2879-3789

Funding

SPIROMICS II: Biological underpinnings of COPD heterogeneity and progressionU01HL137880 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI WOODRUFF, PRESCOTT G · 2017 to 2021
$27.9M
Tissue resident macrophages regulate proteostasis in the aging lungP01AG049665 · NIA · NORTHWESTERN UNIVERSITY AT CHICAGO · PI NAVDEEP S CHANDEL · 2015 to 2026
$26.9M
Technology CoreU19AI135964 · NIAID · NORTHWESTERN UNIVERSITY AT CHICAGO · PI JUSTIN B. STARREN · 2018 to 2026
$24.7M
Lung Macrophage Programming in Acute Lung InjuryR35HL140039 · NHLBI · NATIONAL JEWISH HEALTH · PI JANSSEN, WILLIAM · 2018 to 2024
$6.4M
Mechanisms of lung macrophage programming by MUC5B during health and diseaseR01HL130938 · NHLBI · UNIVERSITY OF COLORADO DENVER · PI EVANS, CHRISTOPHER M · 2016 to 2025
$5.2M
Understanding the unique role endogenous APC subtypes play during the rejection of MAg-mismatched cellsR01HL115334 · NHLBI · NATIONAL JEWISH HEALTH · PI JAKUBZICK, CLAUDIA V · 2013 to 2020
$3.3M
CXCL10/CXCR3 regulation of ozone-induced epithelial permeabilityR01ES027574 · NIEHS · DUKE UNIVERSITY · PI TIGHE, ROBERT MATTHEW · 2018 to 2022
$3.1M
Novel role for CD163 in ozone induced alterations of pulmonary immunityR01ES028829 · NIEHS · OHIO STATE UNIVERSITY · PI GOWDY, KYMBERLY MAE · 2018 to 2022
$2.7M
Functional roles of human pulmonary DC's in the lung draining lymph nodesR01HL135001 · NHLBI · NATIONAL JEWISH HEALTH · PI JAKUBZICK, CLAUDIA V · 2017 to 2020
$2.3M
Regulatory T cell Uhrf1 and DNA methylation in repair of acute lung injuryK08HL128867 · NHLBI · NORTHWESTERN UNIVERSITY AT CHICAGO · PI SINGER, BENJAMIN DAVID · 2015 to 2019
$779k
Regulation of Lung Fibrosis by Alternatively Activated Macrophages and Arginase-1K08HL105537 · NHLBI · DUKE UNIVERSITY · PI TIGHE, ROBERT MATTHEW · 2012 to 2016
$492k
Differential role of tissue-resident and monocyte-derived alveolar macrophages in the development of pulmonary fibrosisR56HL135124 · NHLBI · NORTHWESTERN UNIVERSITY AT CHICAGO · PI MISHARIN, ALEXANDER · 2017 to 2017
$460k
BLRD VA IK2 BX002401CSRD VA I01 CX000911CSRD VA I01 CX001553NHLBI NIH HHS K08 HL105537NHLBI NIH HHS K08 HL128867NHLBI NIH HHS R01 HL115334NHLBI NIH HHS R01 HL130938NHLBI NIH HHS R01 HL135001NHLBI NIH HHS R35 HL140039NHLBI NIH HHS R56 HL135124NHLBI NIH HHS U01 HL137880NIAID NIH HHS U19 AI135964NIA NIH HHS P01 AG049665NIEHS NIH HHS R01 ES027574NIEHS NIH HHS R01 ES028829
6 · The paper itself

Abstract

Defining responses of the structural and immune cells in biologic systems is critically important to understanding disease states and responses to injury. This requires accurate and sensitive methods to define cell types in organ systems. The principal method to delineate the cell populations involved in these processes is flow cytometry. Although researchers increasingly use flow cytometry, technical challenges can affect its accuracy and reproducibility, thus significantly limiting scientific advancements. This challenge is particularly critical to lung immunology, as the lung is readily accessible and therefore used in preclinical and clinical studies to define potential therapeutics. Given the importance of flow cytometry in pulmonary research, the American Thoracic Society convened a working group to highlight issues and technical challenges to the performance of high-quality pulmonary flow cytometry, with a goal of improving its quality and reproducibility.

Indexed as

AnimalsApoptosisCell SeparationCongresses as TopicFlow CytometryHumansLungLung DiseasesMyeloid CellsPhenotypePractice Guidelines as TopicReproducibility of ResultsSocieties, MedicalUnited Statescellsflow cytometrylung biologyreproducibility

Identifiers

PMID31368812
PMCPMC6670040

What OpenQuestion holds

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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.