Evidence map›Paper›PMID 31368061›Full record

ArticleJournal of molecular neuroscience : MN2019

Plasma Exosomal miR-450b-5p as a Possible Biomarker and Therapeutic Target for Transient Ischaemic Attacks in Rats.

XiuMei Luo, Wei Wang, DongBin Li, Chen Xu, Bao Liao, FengMei Li, Xia Zhou, Wu Qin, Jingli Liu

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of molecular neuroscience : MN, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 32 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

XiuMei LuoDepartment of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, 530021, China.
Wei WangDepartment of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, 530021, China.
DongBin LiDepartment of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, 530021, China.
Chen XuDepartment of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, 530021, China.
Bao LiaoDepartment of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, 530021, China.
FengMei LiDepartment of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, 530021, China.
Xia ZhouDepartment of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, 530021, China.
Wu QinDepartment of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, 530021, China.
Jingli LiuDepartment of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, 530021, China. lilicomet@163.com.ORCID http://orcid.org/0000-0002-5375-7491
Guangxi Medical University · CN

Funding

National Natural Science Foundation of China 81660354
6 · The paper itself

Abstract

Transient ischaemic attack (TIA) and cerebral infarction are difficult to identify within the thrombolytic time window. Blood markers are efficient, economical and noninvasive and can be beneficial in the diagnosis of many diseases. Plasma exosomal biomarkers are rarely reported in TIA. Exosomal microRNAs (miRNAs) were extracted from plasma and cerebrospinal fluid after middle cerebral artery occlusion (MCAo) in rats (0 min, 5 min, 10 min, 2 h). Deep sequencing was used to detect exosomal miRNAs in rat plasma and confirm significant differentially expressed miRNAs. Polymerase chain reaction (PCR) was used to detect the differentially expressed miRNAs in plasma and cerebrospinal fluid. Exosomal miRNAs with the same expression trends in plasma and cerebrospinal fluid were selected, and bioinformatics analysis was then carried out. Finally, the area under the curve (AUC) of the receiver operating characteristic (ROC) curve was determined to assess the diagnostic accuracy of miRNAs for TIA in rats. First, high-throughput sequencing was used to detect the expression level of plasma exosome miRNA, and rno-miR-450b-5p with a decreasing expression level was screened. Second, the expression levels of exosomal miRNAs were verified in cerebrospinal fluid and plasma samples by PCR, and the results indicated that exosomal rno-miR-450b-5p was similarly expressed in cerebrospinal fluid and plasma. ROC analysis showed high AUC values for rno-miR-450b-5p (0.880) in the 10 min ischaemia rats compared with the control rats. Finally, bioinformatic analysis indicated that exosomal rno-miR-450b-5p may be involved in cerebral ischaemia. Plasma exosomal rno-miR-450b-5p has a high diagnostic value and may become a therapeutic target for rat TIA.

Indexed as

AnimalsBiomarkersExosomesInfarction, Middle Cerebral ArteryMicroRNAsRatsBiomarkersMicroRNAsMIRN450 microRNA, ratBiomarkerDroplet digital PCRPlasma/cerebrospinal fluid exosomesRno-miR-450b-5pTransient ischaemic attack

Identifiers

PMID31368061
OpenAlexW2964990301

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.