Evidence map›Paper›PMID 31367897›Full record

ArticleMetabolomics : Official journal of the Metabolomic Society2019

Using metabolomics to assess the sub-lethal effects of zinc and boscalid on an estuarine polychaete worm over time.

Georgia M Sinclair, Allyson L O'Brien, Michael Keough, David P De Souza, Saravanan Dayalan, Komal Kanojia, Konstantinos Kouremenos, Dedreia L Tull, Rhys A Coleman, Oliver A H Jones and 1 more

Abstract read
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In one paragraph

Article in Metabolomics : Official journal of the Metabolomic Society, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
3.3field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 51 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 4 countries.

Georgia M SinclairSchool of BioSciences, The University of Melbourne, Royal Parade, Parkville, VIC, 3052, Australia.
Allyson L O'BrienSchool of BioSciences, The University of Melbourne, Royal Parade, Parkville, VIC, 3052, Australia.
Michael KeoughSchool of BioSciences, The University of Melbourne, Royal Parade, Parkville, VIC, 3052, Australia.
David P De SouzaMetabolomics Australia, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, 30 Flemington Road, Parkville, VIC, 3010, Australia.
Saravanan DayalanMetabolomics Australia, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, 30 Flemington Road, Parkville, VIC, 3010, Australia.
Komal KanojiaMetabolomics Australia, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, 30 Flemington Road, Parkville, VIC, 3010, Australia.
Konstantinos KouremenosMetabolomics Australia, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, 30 Flemington Road, Parkville, VIC, 3010, Australia.
Dedreia L TullMetabolomics Australia, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, 30 Flemington Road, Parkville, VIC, 3010, Australia.
Rhys A ColemanMelbourne Water Corporation, 990 La Trobe Street, Docklands, VIC, 3000, Australia.
Oliver A H JonesAustralian Centre for Research on Separation Science (ACROSS), School of Science, RMIT University, GPO Box 2476, Melbourne, VIC, 3001, Australia.ORCID 0000-0002-4541-662X
Sara M LongSchool of BioSciences, The University of Melbourne, Royal Parade, Parkville, VIC, 3052, Australia. sara.long@rmit.edu.au.ORCID 0000-0001-5323-8846
The University of Melbourne · AUCSL (United Kingdom) · GBMelbourne Water · AURMIT University · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionZinc is a heavy metal commonly detected in urban estuaries around Australia. Boscalid is a fungicide found in estuaries, both in water and sediment, it enters the system predominantly through agricultural run-off. Zinc is persistent while boscalid breaks down, with a half-life of 108 days. Both contaminants are widely distributed and their effects on ecosystems are not well understood.

objectivesThe aim of this study was to determine the metabolite changes in Simplisetia aequisetis (an estuarine polychaete) following laboratory exposure to a sub-lethal concentration of zinc or boscalid over a 2-week period.

methodsIndividuals were collected at six time points over a 2-week period. Whole polychaete metabolites were extracted and quantified using a multi-platform approach. Polar metabolites were detected using a semi-targeted GC-MS analysis and amine containing compounds were analysed using a targeted LC-MS analysis. Total lipid energy content was also analysed for Simplisetia aequisetis.

resultsThe pathways that responded to zinc and boscalid exposure were alanine, aspartate and glutamate metabolism (AAG); glycine, serine and threonine metabolism (GST) and metabolites associated with the tricarboxylic acid cycle (TCA). Results showed that changes in total abundance of some metabolites could be detected as early as 24-h exposure. Changes were detected in the metabolites before commonly used total lipid energy assays identified effects.

conclusionA multi-platform approach provided a holistic overview of the metabolomic response to contaminants in polychaetes. This approach shows promise to be used in biomonitoring programs to provide early diagnostic indicators of contamination and exposure.

Indexed as

MetabolomicsAnimalsBiphenyl CompoundsChloridesNiacinamidePolychaetaTime FactorsZinc Compounds2-chloro-N-(4-chlorobiphenyl-2-yl)nicotinamideBiphenyl CompoundsChloridesNiacinamidezinc chlorideZinc CompoundsBoscalidEcotoxicologyEnvironmental metabolomicsInvertebratesTime-courseZinc

Identifiers

PMID31367897
OpenAlexW2965541977

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.