ArticlePlant methods2019
Heterologous overexpression, purification and functional analysis of plant cellulose synthase from green bamboo.
Article in Plant methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed, 7 citations in OpenAlex.
- Transcriptomics Advancement in the Complex Response of Plants to Viroid Infection.International journal of molecular sciences · 2022Review
- Comparative transcriptome analysis elucidates positive physiological effects of foliar application of pyraclostrobin on tomato (Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
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Abstract
backgroundThe cellulose synthase complex (CSC), composed of cellulose synthase (CesA) proteins, is a catalytic enzyme complex involved in cellulose synthesis in the plant cell. CesA proteins synthesize cellulose microfibrils corresponding to the microtubule direction and export linear products across the plasma membrane. However, the CSC arrangement and the mechanism of cellulose synthesis in plant cells remain unclear. Purified CesA proteins are required to determine biochemical and biophysical characteristics.
resultsIn this study, we constructed, expressed, and purified six heterologously expressed cellulose synthases from
conclusionsIn this study, we successfully established a heterologous expression and purification system for BoCesAs. The purified recombinant BoCesA proteins display enzyme activity and can produce protein in milligram quantities for further studies on molecular composition and structure.
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