ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2019
Induction of ALP and MMP9 activity facilitates invasive behavior in heterogeneous human BMSC and HNSCC 3D spheroids.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Adipocytes promoted anoikis resistance of head and neck cancer cells via overexpressed ITGA5.European journal of medical research · 2025Article
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- Three-Dimensional In Vitro Tumor Spheroid Models for Evaluation of Anticancer Therapy: Recent Updates.Cancers · 2023Review
- BMSC-HNC Interaction: Exploring Effects on Bone Integrity and Head and Neck Cancer Progression.International journal of molecular sciences · 2023Article
- Inducible TgfbR1 and Pten deletion in a model of tongue carcinogenesis and chemoprevention.Cancer gene therapy · 2023Article
- Opposing MMP-9 Expression in Mesenchymal Stromal Cells and Head and Neck Tumor Cells after Direct 2D and 3D Co-Culture.International journal of molecular sciences · 2023Article
- Protease-degradable hydrogels with multifunctional biomimetic peptides for bone tissue engineering.Frontiers in bioengineering and biotechnology · 2023Article
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- Integrated analysis of the tumor microenvironment using a reconfigurable microfluidic cell culture platform.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- Human Mesenchymal Stromal Cells Do Not Cause Radioprotection of Head-and-Neck Squamous Cell Carcinoma.International journal of molecular sciences · 2022Article
- Caspase-9 inhibition decreases expression of Mmp9 during chondrogenesis.Histochemistry and cell biology · 2022Article
- Cell Culture-Based Assessment of Toxicity and Therapeutics of Phytochemical Antioxidants.Molecules (Basel, Switzerland) · 2022Review
- Magnetic Compression of Tumor Spheroids Increases Cell Proliferation In Vitro and Cancer Progression In Vivo.Cancers · 2022Article
- Evaluation of aliphatic acid metabolism in bladder cancer with the goal of guiding therapeutic treatment.Frontiers in oncology · 2022Article
- Evaluation of Placental Alkaline Phosphatase Expression as A Potential Target of Solid Tumors Immunotherapy by Using Gene and Protein Expression Repositories.Cell journal · 2021Article
- Safety and Homing of Human Dental Pulp Stromal Cells in Head and Neck Cancer.Stem cell reviews and reports · 2021Article
- Review
- Spheroids and organoids as humanized 3D scaffold-free engineered tissues for SARS-CoV-2 viral infection and drug screening.Artificial organs · 2021Review
- Human Adipose-Derived Stem/Stromal Cells Promote Proliferation and Migration in Head and Neck Cancer Cells.Cancers · 2021Article
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Mesenchymal stem cells (MSCs) are multipotent progenitor cells capable of differentiating into adipocytic, osteogenic, chondrogenic, and myogenic lineages. There is growing evidence that MSCs home into the tumor microenvironment attracted by a variety of signals such as chemokines, growth factors, and cytokines. Tumor-homing stem cells may originate from bone marrow-derived MSCs (BMSCs) or adipose tissue-derived MSCs. Recent scientific data suggest that MSCs in combination with tumor cells can either promote or inhibit tumorigenic behavior. In head and neck squamous cell carcinoma (HNSCC), BMSCs are reported to be enriched with a potential negative role. Here, we evaluated the effect of BMSCs from 4 different donors in combination with 4 HNSCC cell lines in a 3-dimensional multicellular spheroid model. Heterogeneous combinations revealed an up-regulation of gene and protein expression of osteogenic markers runt-related transcription factor 2 (RUNX2) and alkaline phosphatase (ALP) together with a substantial secretion of matrix metalloproteinase 9. Moreover, heterogenous BMSC/tumor spheroids showed increased invasion compared with homogenous spheroids in a Boyden chamber invasion assay. Furthermore, inhibition of ALP resulted in a substantially decreased spreading of heterogeneous spheroids on laminin-rich matrix. In summary, our data suggest a prometastatic effect of BMSCs combined with HNSCC.-Wessely, A., Waltera, A., Reichert, T. E., Stöckl, S., Grässel, S., Bauer, R. J. Induction of ALP and MMP9 activity facilitates invasive behavior in heterogeneous human BMSC and HNSCC 3D-spheroids.
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