Measurement Method Based on Multispectral Three-Dimensional Imaging for the Chlorophyll Contents of Greenhouse Tomato Plants.

Nondestructive plant growth measurement is essential for researching plant growth and health. A nondestructive measurement system to retrieve plant information includes the measurement of morphological and physiological information, but most systems use two independent measurement systems for the two types of characteristics. In this study, a highly integrated, multispectral, three-dimensional (3D) nondestructive measurement system for greenhouse tomato plants was designed. The system used a Kinect sensor, an SOC710 hyperspectral imager, an electric rotary table, and other components. A heterogeneous sensing image registration technique based on the Fourier transform was proposed, which was used to register the SOC710 multispectral reflectance in the Kinect depth image coordinate system. Furthermore, a 3D multiview RGB-D image-reconstruction method based on the pose estimation and self-calibration of the Kinect sensor was developed to reconstruct a multispectral 3D point cloud model of the tomato plant. An experiment was conducted to measure plant canopy chlorophyll and the relative chlorophyll content was measured by the soil and plant analyzer development (SPAD) measurement model based on a 3D multispectral point cloud model and a single-view point cloud model and its performance was compared and analyzed. The results revealed that the measurement model established by using the characteristic variables from the multiview point cloud model was superior to the one established using the variables from the single-view point cloud model. Therefore, the multispectral 3D reconstruction approach is able to reconstruct the plant multispectral 3D point cloud model, which optimizes the traditional two-dimensional image-based SPAD measurement method and can obtain a precise and efficient high-throughput measurement of plant chlorophyll.