Evidence map›Paper›PMID 31364821›Full record

ArticleBrain and behavior2019

Distinct gene alterations between Fos-expressing striatal and thalamic neurons after withdrawal from methamphetamine self-administration.

Xuan Li, Ian R Davis, Olivia M Lofaro, Jianjun Zhang, Raffaello Cimbro, F Javier Rubio

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Brain and behavior, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.3field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 6 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 2 countries.

Xuan LiDepartment of Psychology, University of Maryland College Park, College Park, Maryland.ORCID 0000-0002-6737-1223
Ian R DavisDepartment of Psychology, University of Maryland College Park, College Park, Maryland.
Olivia M LofaroIntramural Research Program, NIDA, NIH, DHHS, Baltimore, Maryland.
Jianjun ZhangCAS Key Laboratory of Mental Health, Institute of Psychology, Beijing, China.ORCID 0000-0001-7526-7234
Raffaello CimbroDivision of Rheumatology, School of Medicine, Johns Hopkins University, Baltimore, Maryland.
F Javier RubioIntramural Research Program, NIDA, NIH, DHHS, Baltimore, Maryland.ORCID 0000-0001-5480-0474
United States Department of Health and Human Services · USUniversity of Maryland, College Park · USInstitute of Psychology, Chinese Academy of Sciences · CNJohns Hopkins University · US

Funding

Phenotype-Specific Immune Responses in the Systemic VasculitidesP30AR053503 · NIAMS · JOHNS HOPKINS UNIVERSITY · PI ROSEN, ANTONY · 2006 to 2015
$6.4M
Circuit and epigenetic mechanisms underlying incubation of methamphetamine cravingR00DA041350 · NIDA · UNIV OF MARYLAND, COLLEGE PARK · PI LI, XUAN ANNA · 2018 to 2020
$747k
NIAMS NIH HHS P30 AR053503NIDA NIH HHS R00 DA041350
6 · The paper itself

Abstract

backgroundMethamphetamine (Meth) seeking progressively increases after withdrawal (incubation of Meth craving). We previously demonstrated a role of anterior intralaminar nucleus of thalamus (AIT) to dorsomedial striatum (DMS) projections in this incubation. Here, we examined molecular alterations in DMS and AIT neurons activated (identified by neuronal activity marker Fos) during "incubated" Meth-seeking relapse test after prolonged withdrawal.

methodsWe trained male rats to self-administer Meth or saline (control condition) for 10 days (6 hr/day). Using fluorescence-activated cell sorting, we examined gene expression in Fos-positive (activated during a 2-hr relapse test) and Fos-negative (nonactivated) DMS and AIT neurons.

resultsIn DMS, we found increased mRNA expressions of immediate early genes (IEGs) (Arc, Egr1, Npas4, Fosb), Trkb, glutamate receptors subunits (Gria3, Grin1, Grin2b, Grm1), and epigenetic enzymes (Hdac3, Hdac5, Crebbp) in Fos-positive neurons, compared with Fos-negative neurons. In AIT, we found that fewer genes (Egr1, Fosb, TrkB, Grin1, and Hdac5) exhibited increased mRNA expression in Fos-positive neurons. Unexpectedly, in both brain regions, gene alterations described above also occurred in drug-naïve saline self-administration control rats.

conclusionsThese results demonstrated that transcriptional regulations in Fos-positive neurons activated during the relapse tests are brain region-specific but are not uniquely associated with drug exposure during the self-administration training.

Indexed as

AnimalsCorpus StriatumCravingDisease Models, AnimalGene Expression RegulationMaleMethamphetamineNeuronsProto-Oncogene Proteins c-fosRatsRats, Sprague-DawleySelf AdministrationSubstance Withdrawal SyndromeThalamusMethamphetamineProto-Oncogene Proteins c-fosanterior intralaminar nucleus of thalamusdorsal striatumfluorescence-activated cell sortingFosincubation of drug cravingmethamphetamine relapse

Identifiers

PMID31364821
PMCPMC6749486
OpenAlexW2965178411

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.