ReviewGigaScience2019
Prospects and challenges of implementing DNA metabarcoding for high-throughput insect surveillance.
Review in GigaScience, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
70 citing papers in PubMed.
- Simultaneous identification of multiple thrips species (Thysanoptera: Thripidae) from sticky traps using DNA metabarcoding.Journal of economic entomology · 2026Article
- eDNA analysis of yard waste samples reveals taxonomical diversity, sequence database limitations, and consistencies across sequencing platforms.Journal of insect science (Online) · 2026Article
- Integrating Megabarcoding and Metabarcoding to Unlock Diversity and Distribution Data Shortfalls in Dark Taxa.Molecular ecology resources · 2026Article
- Comparison of Environmental DNA and Bulk DNA Metabarcoding for Assessing Terrestrial Arthropod Diversity Across Three Habitat Types on Guam.Molecular ecology resources · 2026Article
- Comparative Analysis of Lepidopteran Community Structure Using DNA Metabarcoding: Warm-Temperate Forest Versus Grass-Shrub Ecotones in Pangquangou National Nature Reserve.Ecology and evolution · 2026Article
- Assessing collection and trapping methods for diagnostics and surveillance of psyllids (Hemiptera: Psylloidea) in and around citrus orchards.Journal of economic entomology · 2026Article
- The shared performance of different COI regions increases the niche breadth detectable from bat guano eDNA.Genetics and molecular biology · 2026Article
- Dietary DNA Metabarcoding From Animal Fecal Samples.Current protocols · 2026Article
- Review
- CODABEILLES: a reliable reference library of COI DNA barcodes for French wild bees monitoring (Apoidea: Anthophila).BMC ecology and evolution · 2025Article
- An All-in-One Metabarcoding Approach to Mosquito and Arbovirus Xenosurveillance.Molecular ecology resources · 2025Article
- Effects of land cover and protected areas on flying insect diversity.Conservation biology : the journal of the Society for Conservation Biology · 2025Article
- Use of Multi-Locus Metabarcoding to Inform an Australian Government Biosecurity Response on the Origins of Suspected Illegal Plant Products.International journal of molecular sciences · 2025Article
- Comprehensive Mosquito Wing Image Repository for Advancing Research on Geometric Morphometric- and AI-Based Identification.Scientific data · 2025Article
- Integrating DNA Barcoding Within an Orthogonal Approach for Herbal Product Authentication: A Narrative Review.Phytochemical analysis : PCA · 2025Review
- Viroscope™: a universal solution for plant virus and viroid diagnostics using HTS and cloud-based analysis.Frontiers in microbiology · 2025Article
- Building a reliable 16S mini-barcode library of wild bees from Occitania, south-west of France.Biodiversity data journal · 2025Article
- Phylogenetic and Comparative Genomics Study ofInsects · 2024Article
- The Field Automatic Insect Recognition-Device-A Non-Lethal Semi-Automatic Malaise Trap for Insect Biodiversity Monitoring: Proof of Concept.Ecology and evolution · 2024Article
- Effect of temperature and humidity on insect DNA integrity evaluated by real-time PCR.Journal of economic entomology · 2024Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Trap-based surveillance strategies are widely used for monitoring of invasive insect species, aiming to detect newly arrived exotic taxa as well as track the population levels of established or endemic pests. Where these surveillance traps have low specificity and capture non-target endemic species in excess of the target pests, the need for extensive specimen sorting and identification creates a major diagnostic bottleneck. While the recent development of standardized molecular diagnostics has partly alleviated this requirement, the single specimen per reaction nature of these methods does not readily scale to the sheer number of insects trapped in surveillance programmes. Consequently, target lists are often restricted to a few high-priority pests, allowing unanticipated species to avoid detection and potentially establish populations. DNA metabarcoding has recently emerged as a method for conducting simultaneous, multi-species identification of complex mixed communities and may lend itself ideally to rapid diagnostics of bulk insect trap samples. Moreover, the high-throughput nature of recent sequencing platforms could enable the multiplexing of hundreds of diverse trap samples on a single flow cell, thereby providing the means to dramatically scale up insect surveillance in terms of both the quantity of traps that can be processed concurrently and number of pest species that can be targeted. In this review of the metabarcoding literature, we explore how DNA metabarcoding could be tailored to the detection of invasive insects in a surveillance context and highlight the unique technical and regulatory challenges that must be considered when implementing high-throughput sequencing technologies into sensitive diagnostic applications.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.