ReviewNucleus (Austin, Tex.)2019
Probing the 3D architecture of the plant nucleus with microscopy approaches: challenges and solutions.
Review in Nucleus (Austin, Tex.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 47 citations in OpenAlex.
- SlimVar for rapid in vivo single-molecule tracking of chromatin regulators in plants.Nature communications · 2025Article
- Heat stress response and transposon control in plant shoot stem cells.Plant physiology · 2025Article
- 3D STED Imaging of Isolated Arabidopsis thaliana Nuclei.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Microfluidics to Follow Spatiotemporal Dynamics at the Nucleo-Cytoplasmic Interface During Plant Root Growth.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Connecting high-resolution 3D chromatin maps with cell division and cell differentiation at the root apical meristem.Plant cell reports · 2024Article
- Insight into chromatin compaction and spatial organization in rice interphase nuclei.Frontiers in plant science · 2024Article
- Use of Super-Resolution Live Cell Imaging to Distinguish Endoplasmic Reticulum: Nuclear Envelope Subcellular Localization.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Novel whole-mount FISH analysis for intact root of Arabidopsis thaliana with spatial reference to 3D visualization.Journal of plant research · 2023Article
- Reference genes for quantitative Arabidopsis single molecule RNA fluorescence in situ hybridization.Journal of experimental botany · 2023Article
- Optical imaging of the small intestine immune compartment across scales.Communications biology · 2023Article
- Image analysis workflows to reveal the spatial organization of cell nuclei and chromosomes.Nucleus (Austin, Tex.) · 2022Article
- The INDEPTH (Impact of Nuclear Domains on Gene Expression and Plant Traits) Academy: a community resource for plant science.Journal of experimental botany · 2022Article
- Deep learning-based image processing in optical microscopy.Biophysical reviews · 2022Review
- ROS scavengers decrease γH2ax spots in motor neuronal nuclei of ALS model miceFrontiers in cellular neuroscience · 2022Article
- The Active Segmentation Platform for Microscopic Image Classification and Segmentation.Brain sciences · 2021Article
- Methods of In Situ Quantitative Root Biology.Plants (Basel, Switzerland) · 2021Article
- FISH Going Meso-Scale: A Microscopic Search for Chromatin Domains.Frontiers in cell and developmental biology · 2021Review
- ANCHOR: A Technical Approach to Monitor Single-Copy Locus LocalizationFrontiers in plant science · 2021Article
- Automated 3D bio-imaging analysis of nuclear organization by NucleusJ 2.0.Nucleus (Austin, Tex.) · 2020Article
- 3-D Nucleus Architecture in Oat × Maize Addition Lines.International journal of molecular sciences · 2020Article
Corrections and comments
- Erratum issuedCorrection.2020
Authors and funding
9 authors at 7 institutions in 7 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The eukaryotic cell nucleus is a central organelle whose architecture determines genome function at multiple levels. Deciphering nuclear organizing principles influencing cellular responses and identity is a timely challenge. Despite many similarities between plant and animal nuclei, plant nuclei present intriguing specificities. Complementary to molecular and biochemical approaches, 3D microscopy is indispensable for resolving nuclear architecture. However, novel solutions are required for capturing cell-specific, sub-nuclear and dynamic processes. We provide a pointer for utilising high-to-super-resolution microscopy and image processing to probe plant nuclear architecture in 3D at the best possible spatial and temporal resolution and at quantitative and cell-specific levels. High-end imaging and image-processing solutions allow the community now to transcend conventional practices and benefit from continuously improving approaches. These promise to deliver a comprehensive, 3D view of plant nuclear architecture and to capture spatial dynamics of the nuclear compartment in relation to cellular states and responses.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.