ArticleMolecular microbiology2019
Identification, characterization and benefits of an exclusion system in an integrative and conjugative element of Bacillus subtilis.
Article in Molecular microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- The extended mobility of plasmids.Nucleic acids research · 2025Review
- Activation and modulation of the host response to DNA damage by an integrative and conjugative element.Journal of bacteriology · 2025Article
- It's complicated: relationships between integrative and conjugative elements and their bacterial hosts.Current opinion in microbiology · 2024Review
- Activation and modulation of the host response to DNA damage by an integrative and conjugative element.bioRxiv : the preprint server for biology · 2024Article
- Surface exclusion of IncC conjugative plasmids and their relatives.PLoS genetics · 2024Article
- Contribution of the Mobilome to the Configuration of the Resistome ofInternational journal of molecular sciences · 2024Article
- Characterization of ConE, the VirB4 Homolog of the Integrative and Conjugative Element ICEJournal of bacteriology · 2023Article
- Conjugation's Toolkit: the Roles of Nonstructural Proteins in Bacterial Sex.Journal of bacteriology · 2023Review
- Second-Generation Transfer Mediates Efficient Propagation of ICEJournal of bacteriology · 2022Article
- Activation of the integrative and conjugative element Tn916 causes growth arrest and death of host bacteria.PLoS genetics · 2022Article
- A CRISPR interference screen reveals a role for cell wall teichoic acids in conjugation in Bacillus subtilis.Molecular microbiology · 2022Article
- Biology and engineering of integrative and conjugative elements: Construction and analyses of hybrid ICEs reveal element functions that affect species-specific efficiencies.PLoS genetics · 2022Article
- Interactions between mobile genetic elements: An anti-phage gene in an integrative and conjugative element protects host cells from predation by a temperate bacteriophage.PLoS genetics · 2022Article
- Cell Factory Engineering of UndomesticatedFrontiers in microbiology · 2022Article
- Specificity and Selective Advantage of an Exclusion System in the Integrative and Conjugative Element ICEJournal of bacteriology · 2021Article
- Mobile Type VI secretion system loci of the gut Bacteroidales display extensive intra-ecosystem transfer, multi-species spread and geographical clustering.PLoS genetics · 2021Article
- Article
- The Large pBS32/pLS32 Plasmid of Ancestral Bacillus subtilis.Journal of bacteriology · 2020Review
- Symbiont-Mediated Insecticide Detoxification as an Emerging Problem in Insect Pests.Frontiers in microbiology · 2020Review
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3 authors.
Funding
Abstract
Integrative and conjugative elements (ICEs) are mobile genetic elements that transfer from cell to cell by conjugation (like plasmids) and integrate into the chromosomes of bacterial hosts (like lysogenic phages or transposons). ICEs are prevalent in bacterial chromosomes and play a major role in bacterial evolution by promoting horizontal gene transfer. Exclusion prevents the redundant transfer of conjugative elements into host cells that already contain a copy of the element. Exclusion has been characterized mostly for conjugative elements of Gram-negative bacteria. Here, we report the identification and characterization of an exclusion mechanism in ICEBs1 from the Gram-positive bacterium Bacillus subtilis. We found that cells containing ICEBs1 inhibit the activity of the ICEBs1-encoded conjugation machinery in other cells. This inhibition (exclusion) was specific to the cognate conjugation machinery and the ICEBs1 gene yddJ was both necessary and sufficient to mediate exclusion by recipient cells. Through a mutagenesis and enrichment screen, we identified exclusion-resistant mutations in the ICEBs1 gene conG. Using genes from a heterologous but related ICE, we found that the exclusion specificity was determined by ConG and YddJ. Finally, we found that under conditions that support conjugation, exclusion provides a selective advantage to the element and its host cells.
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